Thomas Hurley

  • 4871 Citations
  • 40 h-Index
1979 …2021
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Publications 1990 2019

  • 4871 Citations
  • 40 h-Index
  • 104 Article
  • 2 Chapter
  • 1 Editorial
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Article
1990
47 Citations (Scopus)
Alcohol Dehydrogenase
Amino Acid Substitution
Substitution reactions
Amino Acids
Kinetics
1991
2 Citations (Scopus)

Characterization of human alcohol dehydrogenases containing substitutions at amino acids 47 and 51

Hurley, T., Ehrig, T., Edenberg, H. & Bosron, W. F., 1991, In : Advances in Experimental Medicine and Biology. 284, p. 271-275 5 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Amino Acid Substitution
Site-Directed Mutagenesis
Recombinant Proteins
Histidine
46 Citations (Scopus)

General Base Catalysis in a Glutamine for Histidine Mutant at Position 51 of Human Liver Alcohol Dehydrogenase

Ehrig, T., Hurley, T., Edenberg, H. & Bosron, W. F., 1991, In : Biochemistry. 30, 4, p. 1062-1068 7 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Glutamine
Catalysis
Histidine
Liver
76 Citations (Scopus)

Structure of human β1β1 alcohol dehydrogenase: Catalytic effects of non-active-site substitutions

Hurley, T., Bosron, W. F., Hamilton, J. A. & Mario Amzel, L., 1991, In : Proceedings of the National Academy of Sciences of the United States of America. 88, 18, p. 8149-8153 5 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Coenzymes
NAD
Horses
Enzymes
1992
9 Citations (Scopus)

Crystallization and preliminary X-ray investigation of bovine liver mitochondrial aldehyde dehydrogenase

Hurley, T. & Weiner, H., Oct 20 1992, In : Journal of Molecular Biology. 227, 4, p. 1255-1257 3 p.

Research output: Contribution to journalArticle

Aldehyde Dehydrogenase
Liver Mitochondrion
Crystallization
X-Ray Diffraction
NAD
16 Citations (Scopus)
Alcohol Dehydrogenase
Alcoholism
Alcohols
Amino Acids
Oxidation
2 Citations (Scopus)

Tryptophan fluorescence quenching by alkaline pH and ternary complex formation in human β1β1 and horse EE alcohol dehydrogenases

Ehrig, T., Muhoberac, B. B., Hurley, T. & Bosron, W. F., Apr 6 1992, In : FEBS Letters. 300, 3, p. 283-285 3 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Tryptophan
Horses
Quenching
Fluorescence
1993

Human alcohol dehydrogenase: Design of a secondary alcohol dehydrogenase using crystallography and mutagenesis

Hurley, T., Bosron, W. F. & Mario Amzel, L., 1993, In : Protein Engineering, Design and Selection. 6, p. 44 1 p.

Research output: Contribution to journalArticle

Mutagenesis
Crystallography
Alcohol Dehydrogenase
isopropanol dehydrogenase (NADP)
1994
135 Citations (Scopus)

Catalytic efficiency of human alcohol dehydrogenases for retinol oxidation and retinal reduction

Yang, Z. N., Davis, G. J., Hurley, T., Stone, C. L., Li, T. K. & Bosron, W. F., 1994, In : Alcoholism: Clinical and Experimental Research. 18, 3, p. 587-591 5 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Vitamin A
Oxidation
Isoenzymes
Retinoids
20 Citations (Scopus)

Comparative Roles of Histidine-51 in Human β1β1 and Threonine-51 in ππ Alcohol Dehydrogenases

Davis, G. J., Carr, L. G., Hurley, T., Li, T. K. & Bosron, W. F., Jun 1994, In : Archives of Biochemistry and Biophysics. 311, 2, p. 307-312 6 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Threonine
Histidine
Ethanol
Protons
68 Citations (Scopus)

Structures of three human β alcohol dehydrogenase variants: Correlations with their functional differences

Hurley, T., Bosron, W. F., Stone, C. L. & Amzel, L. M., 1994, In : Journal of Molecular Biology. 239, 3, p. 415-429 15 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Coenzymes
NAD
Enzymes
Enzymes and Coenzymes
1995
22 Citations (Scopus)

Cimetidine inhibition of human gastric and liver alcohol dehydrogenase isoenzymes: Identification of inhibitor complexes by kinetics and molecular modeling

Stone, C. L., Hurley, T., Peggs, C. F., Kedishvili, N. Y., Davis, G. J., Thomasson, H. R., Li, T. K. & Bosron, W. F., 1995, In : Biochemistry. 34, 12, p. 4008-4014 7 p.

Research output: Contribution to journalArticle

Molecular modeling
Alcohol Dehydrogenase
Cimetidine
Liver
Isoenzymes
2 Citations (Scopus)

Cloning and expression of a human stomach alcohol dehydrogenase isozyme

Kedishvili, N. Y., Bosron, W. F., Stone, C. L., Peggs, C. F., Thomasson, H. R., Popov, K. M., Carr, L. G., Hurley, T., Edenberg, H. & Li, T. K., 1995, In : Advances in Experimental Medicine and Biology. 372, p. 341-347 7 p.

Research output: Contribution to journalArticle

Molecular Sequence Data
Bacteriophage lambda
Amino Acid Sequence Homology
Oligonucleotide Probes
Alcohol Dehydrogenase
75 Citations (Scopus)

Expression and kinetic characterization of recombinant human stomach alcohol dehydrogenase: Active-site amino acid sequence explains substrate specificity compared with liver isozymes

Kedishvili, N. Y., Bosron, W. F., Stone, C. L., Hurley, T., Peggs, C. F., Thomasson, H. R., Popov, K. M., Carr, L. G., Edenberg, H. & Li, T. K., Feb 24 1995, In : Journal of Biological Chemistry. 270, 8, p. 3625-3630 6 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Substrate Specificity
Liver
Isoenzymes
Amino Acid Sequence
5 Citations (Scopus)

The role of leucine 116 in determining substrate specificity in human B1 alcohol dehydrogenase

Hurley, T. & Vessell, D. L., 1995, In : Advances in Experimental Medicine and Biology. 372, p. 321-325 5 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Substrate Specificity
Site-Directed Mutagenesis
Recombinant Proteins
Leucine
1996
98 Citations (Scopus)

Three-dimensional structure of mammalian casein kinase I: Molecular basis for phosphate recognition

Longenecker, K. L., Roach, P. & Hurley, T., Apr 5 1996, In : Journal of Molecular Biology. 257, 3, p. 618-631 14 p.

Research output: Contribution to journalArticle

Casein Kinase I
Phosphates
Catalytic Domain
Adenosine Triphosphate
Protein Kinases
39 Citations (Scopus)

X-ray structure of human β3β3 alcohol dehydrogenase. The contribution of ionic interactions to coenzyme binding

Davis, G. J., Bosron, W. F., Stone, C. L., Owusu-Dekyi, K. & Hurley, T., 1996, In : Journal of Biological Chemistry. 271, 29, p. 17057-17061 5 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Coenzymes
NAD
X-Rays
X rays
1997
32 Citations (Scopus)

cDNA sequence and catalytic properties of a chick embryo alcohol dehydrogenase that oxidizes retinol and 3β,5α-hydroxysteroids

Kedishvili, N. Y., Cough, W. H., Chernoff, E. A. G., Hurley, T., Stone, C. L., Bowman, K. D., Popov, K. M., Bosron, W. F. & Li, T. K., Mar 14 1997, In : Journal of Biological Chemistry. 272, 11, p. 7494-7500 7 p.

Research output: Contribution to journalArticle

Hydroxysteroids
Alcohol Dehydrogenase
Chick Embryo
Vitamin A
Complementary DNA
74 Citations (Scopus)

Structure of human χχ alcohol dehydrogenase: A glutathione-dependent formaldehyde dehydrogenase

Yang, Z. N., Bosron, W. F. & Hurley, T., Jan 1 1997, In : Journal of Molecular Biology. 265, 3, p. 330-343 14 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Glutathione
Zinc
Hydroxy Acids
NAD
264 Citations (Scopus)

Structure of mitochondrial aldehyde dehydrogenase: The genetic component of ethanol aversion

Steinmetz, C. G., Xie, P., Weiner, H. & Hurley, T., May 15 1997, In : Structure. 5, 5, p. 701-711 11 p.

Research output: Contribution to journalArticle

NAD
Ethanol
Niacinamide
Alcoholism
Catalytic Domain
82 Citations (Scopus)

Studies on the regulation of the mitochondrial α-ketoacid dehydrogenase complexes and their kinases

Harris, R., Hawes, J. W., Popov, K. M., Zhao, Y., Shimomura, Y., Sato, J., Jaskiewicz, J. & Hurley, T., 1997, In : Advances in Enzyme Regulation. 37, p. 271-293 23 p.

Research output: Contribution to journalArticle

3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Phosphorylation
Oxidoreductases
Phosphotransferases
Liver
67 Citations (Scopus)

The potential roles of the conserved amino acids in human liver mitochondrial aldehyde dehydrogenase

Sheikh, S., Ni, L., Hurley, T. & Weiner, H., 1997, In : Journal of Biological Chemistry. 272, 30, p. 18817-18822 6 p.

Research output: Contribution to journalArticle

Aldehyde Dehydrogenase
Esterases
Aldehydes
Liver
NAD
13 Citations (Scopus)

Three-dimensional structures of human alcohol dehydrogenase isoenzymes reveal the molecular basis for their functional diversity

Hurley, T., Steinmetz, C. G., Xie, P. & Yang, Z. N., 1997, In : Advances in Experimental Medicine and Biology. 414, p. 291-302 12 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
X ray crystallography
Recombinant Proteins
Escherichia coli
Isoenzymes
71 Citations (Scopus)

X-ray structure of human class IV σσ alcohol dehydrogenase. Structural basis for substrate specificity

Xie, P., Parsons, S. H., Speckhard, D. C., Bosron, W. F. & Hurley, T., 1997, In : Journal of Biological Chemistry. 272, 30, p. 18558-18563 6 p.

Research output: Contribution to journalArticle

Substrate Specificity
Isoenzymes
X-Rays
Alcohol Dehydrogenase
X rays
1998
20 Citations (Scopus)
Casein Kinase I
Catalytic Domain
Dimers
Protein Kinases
Escherichia coli
1999
27 Citations (Scopus)

Different mutations in the same codon of the proteolipid protein gene, PLP, may help in correlating genotype with phenotype in Pelizaeus-Merzbacher disease/X-linked spastic paraplegia (PMD/SPG2)

Hodes, M. E., Zimmerman, A. W., Aydanian, A., Naidu, S., Miller, N. R., Garcia Oller, J. L., Barker, B., Aleck, K. A., Hurley, T. & Dlouhy, S., Jan 15 1999, In : American Journal of Medical Genetics. 82, 2, p. 132-139 8 p.

Research output: Contribution to journalArticle

Pelizaeus-Merzbacher Disease
Proteolipids
Paraplegia
Codon
Genotype
4 Citations (Scopus)

Evaluation of the roles of the conserved residues of aldehyde dehydrogenase

Hurley, T. & Weiner, H., 1999, In : Advances in Experimental Medicine and Biology. 463, p. 45-52 8 p.

Research output: Contribution to journalArticle

Recombinant Fusion Proteins
Mutagenesis
Aldehyde Dehydrogenase
Conserved Sequence
Threonine
63 Citations (Scopus)

Human liver mitochondrial aldehyde dehydrogenase: Three-dimensional structure and the restoration of solubility and activity of chimeric forms

Ni, L., Zhou, J., Hurley, T. & Weiner, H., 1999, In : Protein Science. 8, 12, p. 2784-2790 7 p.

Research output: Contribution to journalArticle

Aldehyde Dehydrogenase
Liver
Solubility
Restoration
Isoenzymes
28 Citations (Scopus)

Methionine-141 directly influences the binding of 4-methylpyrazole in human σσ alcohol dehydrogenase

Xie, P. T. & Hurley, T., 1999, In : Protein Science. 8, 12, p. 2639-2644 6 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Methionine
Isoenzymes
Enzymes
Mutagenesis
5 Citations (Scopus)

The pH-dependent binding of NADH and subsequent enzyme isomerization of human liver β3β3 alcohol dehydrogenase

Stone, C. L., Jipping, M. B., Owusu-Dekyi, K., Hurley, T., Li, T. K. & Bosron, W. F., May 4 1999, In : Biochemistry. 38, 18, p. 5829-5835 7 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Isomerization
Liver
NAD
Enzymes
20 Citations (Scopus)

Three-dimensional structure of mitochondrial aldehyde dehydrogenase: Mechanistic implications

Hurley, T., Steinmetz, C. G. & Weiner, H., 1999, In : Advances in Experimental Medicine and Biology. 463, p. 15-25 11 p.

Research output: Contribution to journalArticle

Aldehyde Dehydrogenase
Protein Conformation
Mitochondria
X ray crystallography
X Ray Crystallography
2000
17 Citations (Scopus)
Aldehyde Dehydrogenase
Liver
NAD
Arginine
Mutation
52 Citations (Scopus)
Protein Phosphatase 1
Protein Binding
Inhibitory Concentration 50
Peptides
Phosphoprotein Phosphatases
48 Citations (Scopus)

Kinetic mechanism of human glutathione-dependent formaldehyde dehydrogenase

Sanghani, P. C., Stone, C. L., Ray, B. D., Pindel, E. V., Hurley, T. & Bosron, W. F., Sep 5 2000, In : Biochemistry. 39, 35, p. 10720-10729 10 p.

Research output: Contribution to journalArticle

glutathione-independent formaldehyde dehydrogenase
Glutathione
Formaldehyde
Kinetics
formic acid
2001
26 Citations (Scopus)

Order and disorder in mitochondrial aldehyde dehydrogenase

Hurley, T., Perez-Miller, S. & Breen, H., Jan 30 2001, In : Chemico-Biological Interactions. 130-132, p. 3-14 12 p.

Research output: Contribution to journalArticle

Aldehyde Dehydrogenase
Coenzymes
Enzymes
Dimers
NAD
43 Citations (Scopus)

Three-dimensional structures of the three human class I alcohol dehydrogenases

Niederhut, M. S., Gibbons, B. J., Perez-Miller, S. & Hurley, T., 2001, In : Protein Science. 10, 4, p. 697-706 10 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Isoenzymes
Genes
Substrates
Substitution reactions
2002
92 Citations (Scopus)

Crystal structure of the autocatalytic initiator of glycogen biosynthesis, glycogenin

Gibbons, B. J., Roach, P. & Hurley, T., 2002, In : Journal of Molecular Biology. 319, 2, p. 463-477 15 p.

Research output: Contribution to journalArticle

Glycogen
Uridine Diphosphate Glucose
Glucose
Glycosyltransferases
4 alpha-glucanotransferase
43 Citations (Scopus)

Human glutathione-dependent formaldehyde dehydrogenase. Structural changes associated with ternary complex formation

Sanghani, P. C., Bosron, W. F. & Hurley, T., Dec 24 2002, In : Biochemistry. 41, 51, p. 15189-15194 6 p.

Research output: Contribution to journalArticle

glutathione-independent formaldehyde dehydrogenase
Coenzymes
Glutathione
Zinc
Catalytic Domain
56 Citations (Scopus)

Human glutathione-dependent formaldehyde dehydrogenase. Structures of apo, binary, and inhibitory ternary complexes

Sanghani, P. C., Robinson, H., Bosron, W. F. & Hurley, T., Sep 3 2002, In : Biochemistry. 41, 35, p. 10778-10786 9 p.

Research output: Contribution to journalArticle

glutathione-independent formaldehyde dehydrogenase
Glutathione
Zinc
Catalytic Domain
Substrates
16 Citations (Scopus)

Lessons from a bacterial cocaine esterase

Bosron, W. F. & Hurley, T., 2002, In : Nature Structural Biology. 9, 1, p. 4-5 2 p.

Research output: Contribution to journalArticle

Catalytic Antibodies
Bacterial Structures
Carboxylesterase
Cocaine
Toxicity
61 Citations (Scopus)

Multiple conformations of NAD and NADH when bound to human cytosolic and mitochondrial aldehyde dehydrogenase

Hammen, P. K., Allali-Hassani, A., Hallenga, K., Hurley, T. & Weiner, H., Jun 4 2002, In : Biochemistry. 41, 22, p. 7156-7168 13 p.

Research output: Contribution to journalArticle

Aldehyde Dehydrogenase
Niacinamide
NAD
Conformations
Coenzymes
2003
122 Citations (Scopus)

Coenzyme isomerization is integral to catalysis in aldehyde dehydrogenase

Perez-Miller, S. J. & Hurley, T., Jun 17 2003, In : Biochemistry. 42, 23, p. 7100-7109 10 p.

Research output: Contribution to journalArticle

Aldehyde Dehydrogenase
Coenzymes
Isomerization
Catalysis
NAD
17 Citations (Scopus)

Structure-function relationships in human Class III alcohol dehydrogenase (formaldehyde dehydrogenase)

Sanghani, P. C., Robinson, H., Bennett-Lovsey, R., Hurley, T. & Bosron, W. F., Feb 1 2003, In : Chemico-Biological Interactions. 143-144, p. 195-200 6 p.

Research output: Contribution to journalArticle

glutathione-independent formaldehyde dehydrogenase
Zinc
Catalytic Domain
Apoenzymes
Alcohols
2004
78 Citations (Scopus)

Identification and analysis of single-nucleotide polymorphisms in the gemcitabine pharmacologic pathway

Fukunaga, A. K., Marsh, S., Murry, D. J., Hurley, T. & McLeod, H. L., 2004, In : Pharmacogenomics Journal. 4, 5, p. 307-314 8 p.

Research output: Contribution to journalArticle

gemcitabine
Single Nucleotide Polymorphism
Gene Frequency
Databases
Population
135 Citations (Scopus)

Methylphenidate is stereoselectively hydrolyzed by human carboxylesterase CES1A1

Sun, Z., Murry, D. J., Sanghani, S. P., Davis, W. I., Kedishvili, N. Y., Zou, Q., Hurley, T. & Bosron, W. F., Aug 2004, In : Journal of Pharmacology and Experimental Therapeutics. 310, 2, p. 469-476 8 p.

Research output: Contribution to journalArticle

Carboxylesterase
Methylphenidate
Isoenzymes
Carboxylic Ester Hydrolases
Sf9 Cells
19 Citations (Scopus)

Structure of three class I human alcohol dehydrogenases complexed with isoenzyme specific formamide inhibitors

Gibbons, B. J. & Hurley, T., Oct 5 2004, In : Biochemistry. 43, 39, p. 12555-12562 8 p.

Research output: Contribution to journalArticle

Alcohol Dehydrogenase
Isoenzymes
Protein Isoforms
Formamides
Zinc
6 Citations (Scopus)

Suicide gene therapy: Conversion of ethanol to acetaldehyde mediated by human beta 2 alcohol dehydrogenase

Savage, P., Cowburn, P., Clemens, D., Hurley, T., Laguda, B., Martin-Duque, P., Vassaux, G. & Lemoine, N. R., Dec 2004, In : Cancer Gene Therapy. 11, 12, p. 774-781 8 p.

Research output: Contribution to journalArticle

Gene Conversion
Acetaldehyde
Genetic Therapy
Suicide
Ethanol
2005
25 Citations (Scopus)

Differential effects of Mg2+ ions on the individual kinetic steps of human cytosolic and mitochondrial aldehyde dehydrogenases

Ho, K. K., Allali-Hassani, A., Hurley, T. & Weiner, H., Jun 7 2005, In : Biochemistry. 44, 22, p. 8022-8029 8 p.

Research output: Contribution to journalArticle

Aldehyde Dehydrogenase
Isoenzymes
Ions
NAD
Kinetics
60 Citations (Scopus)

Disruption of the coenzyme binding site and dimer interface revealed in the crystal structure of mitochondrial aldehyde dehydrogenase "Asian" variant

Larson, H. N., Weiner, H. & Hurley, T., Aug 26 2005, In : Journal of Biological Chemistry. 280, 34, p. 30550-30556 7 p.

Research output: Contribution to journalArticle

Aldehyde Dehydrogenase
Coenzymes
Dimers
Crystal structure
Binding Sites
103 Citations (Scopus)

Hydrolysis of capecitabine to 5′-deoxy-5-fluorocytidine by human carboxylesterases and inhibition by loperamide

Quinney, S., Sanghani, S. P., Davis, W. I., Hurley, T., Sun, Z., Murry, D. J. & Bosron, W. F., Jun 2005, In : Journal of Pharmacology and Experimental Therapeutics. 313, 3, p. 1011-1016 6 p.

Research output: Contribution to journalArticle

Carboxylic Ester Hydrolases
Loperamide
Hydrolysis
Carboxylesterase
Fluorouracil