LIVER GLYCOGEN METABOLISM AND ITS CONTROL BY HORMONES

Project: Research project

Description

The research will evaluate the role of the multiple covalent
phosphorylation of liver glycogen synthase in mediating controls of its
activity by glucose, insulin, glucagon, catecholamines (both as
Alpha-adrenergic and Beta-adrenergic agonists), vasopressin and angiotensin
II. Using purified enzymes, we will characterize and map the sites on
glycogen synthase phosphorylated by some seven different kinases available
to us, including cAMP-dependent protein kinase, two Ca++ dependent protein
kinases and four "independent" protein kinases. The effects of such
phosphorylations on enzyme activity will be determined. By the use of
isolated hepatocytes incubated with 32Pi, antibody techniques for the rapid
isolation of glycogen synthase and peptide mapping of the purified protein,
we will analyze which phosphorylation sites are phosphorylated in whole
cells and which sites are linked to the actions of the above mentioned
hormones. This study will provide important information on the possible
mechanisms of the hormonal control of hepatic glycogen synthesis.
StatusFinished
Effective start/end date8/1/807/31/87

Funding

  • National Institutes of Health

Fingerprint

Glycogen Synthase
Adrenergic beta-Agonists
Liver Glycogen
Hormones
Peptide Mapping
Enzymes
Cyclic AMP-Dependent Protein Kinases
Glucagon
Vasopressins
Protein Kinases
Catecholamines
Hepatocytes
Phosphotransferases
Phosphorylation
Insulin
Glucose
Antibodies
Research
Proteins

Keywords

  • Medicine(all)