MOLECULAR IMMUNOLOGY OF STDS CAUSED BY CHLAMYDIA

  • Batteiger, Byron (PI)
  • Demars, Robert (PI)
  • Niece, Ronald (PI)
  • De Mars, Robert Ivan (PI)
  • Arno, Janet (PI)
  • Jones, Robert (PI)
  • Byrne, Gerald Irwin (PI)
  • Arno, Janet, (PI)

Project: Research project

Description

The goal os this program project is to combine appropriate working teams
immunologists, molecular geneticists, microbiologists and clinicians to
study the molecular immunology of sexually transmitted diseases caused
by Chlamydia trachomatis. The decision to work on a single important STD
pathogen was deliberate effort to blend talents from a variety of
disciplines in such a way to make it possible to comprehensively evaluate
the overall immune response to C. trachomatis, especially in the context
of progress from uncomplicated lower genital tract infections to sequelae
of chronic upper genital tract disease, including involuntary infertility
and adverse outcomes of pregnancy in women. Studies are proposed to
evaluate, at the molecular level the requirements for processing and
presentation of chlamydial antigens, and identification of relevant T
cell epitopes (project 1, DeMars) and the nature and function of antigen
presenting cells in vivo at the site of upper genital tract disease
(project 2, Arno and Bick). Work also is proposed to define the process
of chlamydial persistence in human polarized epithelial cells, including
primary endometrial cells; how immune reactivity contributes to this
process and the molecular characterization of chlamydia as they exist in
the persistent state (project 3, Byrne). Finally, work is proposed to
determine if protective immunity occurs in individuals who recover from
lower genital tract disease; and if so define the requirements based on
epitopic specificity of antibody responses that result in protection
(project 4, Batteiger). These projects will be served by an
administrative core in Madison (Byrne), a clinical core in Indianapolis
(Jones) and a peptide synthesis core in Madison (Niece). The overall aim
of comprehensively increasing our understanding of immunity to chlamydial
genital tract disease will be accomplished by closely networking each of
the four research projects with each other, with the core facilities and
with collaborators who are directly involved in complementary research
programs via STD Center grants. It is anticipated that this focused,
comprehensive approach will serve to increase our knowledge in this field
in a way that has been difficult because of the lack of coordination of
work done in this area. This program project will reduce fragmentation
of effort and facilitate a multicenter cooperative effort with clear
goals in mind.
StatusFinished
Effective start/end date9/1/9312/31/00

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Chlamydia
Humoral Immunity
Epitopes
Membrane Proteins
Recurrence
Adaptive Immunity
Monoclonal Antibodies
Sexually Transmitted Diseases
Infection
Peptides
Cell Culture Techniques
Immunoassay
Serum
Epitope Mapping
Eye Infections
Chlamydia trachomatis
Proteins
Antibody Formation
Serogroup
Serotyping

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)