PATHOGENESIS OF HAEMOPHILUS DUCREYI INFECTIONS

Project: Research project

Description

Haemophilus ducreyi, the etiologic agent of chancroid, is a major
cause of genital ulcers worldwide and is increasing in prevalence
in the United States. Infection with H. ducreyi appears to
increase the transmission of the human immunodeficiency virus in
populations where both diseases are endemic. There is no
information identifying the surface components of the organism
that allow it to invade epithelial surfaces and cause ulcers.
The long term goal of this project is to examine the hypothesis
that the major outer membrane protein (OMP) play a role in the
pathogenesis of infections due to H. ducreyi. The immediate goal
is to examine whether the major OMP has potential as an immune
target and to examine several strains for conserved epitopes on
this protein. To begin to elucidate the structure and function
of the major OMP, the specific aims of this application include:
1) purification and analysis of the major OMP from a recent H.
ducreyi isolate; 2) development of immunologic reagents specific
for the major OMP; 3) cloning and sequencing the gene encoding
the major OMP; 4) localization of epitopes on the major OMP; 5)
characterization of the human immune response to the major OMP;
6) evaluation of a mouse model of H. ducreyi infection and
examining whether antibodies directed against the major OMP
prevent infection in this model.
StatusFinished
Effective start/end date1/1/908/31/15

Funding

  • National Institutes of Health: $256,295.00
  • National Institutes of Health: $113,447.00
  • National Institutes of Health: $375,239.00
  • National Institutes of Health: $335,250.00
  • National Institutes of Health: $50,726.00
  • National Institutes of Health: $53,402.00
  • National Institutes of Health: $335,250.00
  • National Institutes of Health: $275,826.00
  • National Institutes of Health: $662,829.00
  • National Institutes of Health: $313,690.00
  • National Institutes of Health
  • National Institutes of Health: $102,494.00
  • National Institutes of Health: $395,819.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $252,435.00
  • National Institutes of Health: $615,790.00
  • National Institutes of Health: $313,200.00
  • National Institutes of Health: $310,574.00
  • National Institutes of Health

Fingerprint

Haemophilus ducreyi
Haemophilus Infections
Membrane Proteins
Chancroid
Virulence
Infection
Ulcer
Epitopes
Endemic Diseases
HIV

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)