PANCREATIC CANCER AND EPIDERMAL GROWTH FACTOR RECEPTOR

  • Korc, Murray, (PI)

Project: Research project

Description

The exocrine pancreas consists of acinar cells that synthesize and secrete
digestive enzymes, and duct cells that produce bicarbonate-rich fluid.
Pancreatic malignancies may arise from either cell type. The molecular
signals that lead to malignant transformation and subsequent growth
advantage of these cells are not known. Recent studies indicate, however,
that several cultured human pancreatic carcinoma cells have an
overabundance of EGF receptors. The gene coding for the EGF receptor is a
protooncogene that has been implicated in the process of tumor promotion.
In the present proposal we plan to examine the molecular mechanisms that
lead to an overabundance of EGF receptors in cultured human pancreatic
carcinoma cells. Two approaches will be used. First, chromosomal analyses
of seven distinct pancreatic cancer cells will be carried out in order to
look for abnormalities in the region of chromosome 7 that is known to code
for the human EGF receptor gene. Second, appropriate cDNA probes will be
used to determine whether the cell lines that overexpress the EGF receptor
exhibit an increased number of EGF receptor gene copies, or enhanced
stability of its mRNA. The relevance of these observations to pancreatic
cancer in humans will be evaluated by determining the frequency of
overabundance of EGF receptors in biopsy specimen from pancreatic
malignancies. A second important goal will be to study EGF receptor
metabolism and phosphorylation in a pancreatic cancer cell line (Panc-I)
that avidly binds and internalizes EGF without readily degrading the ligand.
StatusFinished
Effective start/end date4/1/8611/30/06

Funding

  • National Institutes of Health
  • National Institutes of Health: $249,664.00
  • National Institutes of Health: $264,950.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $263,248.00
  • National Institutes of Health: $75,200.00
  • National Institutes of Health: $249,664.00
  • National Institutes of Health: $63,514.00
  • National Institutes of Health: $267,318.00
  • National Institutes of Health
  • National Institutes of Health: $248,570.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $166,983.00
  • National Institutes of Health: $245,049.00
  • National Institutes of Health
  • National Institutes of Health: $195,048.00
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Pancreatic Neoplasms
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Transforming Growth Factor alpha
Neoplasms
Cell Line
Growth
Acinar Cells
Adenocarcinoma
Anoikis
Genes
Exocrine Pancreas
Chronic Pancreatitis
Chromosomes, Human, Pair 7
erbB-1 Genes
Bicarbonates
Complementary DNA
ErbB Receptors
Transforming Growth Factor beta
ErbB-3 Receptor

Keywords

  • Medicine(all)