SIGNIFICANCE OF FOLATE BINDING PROTEINS IN HUMAN MILK

Project: Research project

Description

This proposal will focus on two major aspects of the particulate and
soluble folate binding proteins of human milk to identify (1) the factors
involved in determining their quantitative and qualitative content in milk,
and (2) the functional role of these folate binding proteins in the uptake
of folates in the intestine. Studies on the biosynthesis of particulate folate binding proteins with
respect to processing of the polypeptide backbone, glycosylation and
acylation that may have a direct bearing on the membrane insertion, folate
binding capacity and possible conversion to the soluble folate binding
protein will be investigated in cultured mammary cells. The effect of
folate deficiency on the secretion of these folate binding proteins will be
investigated in lactating rats. The influence of various components of human milk, saliva, gastric,
pancreatic and intestinal secretions on the structural integrity and ligand
binding capacity of these proteins and their role in the uptake of folates
will be identified in rat intestinal cells and brush border vesicles. In
addition, the possibility of spontaneous incorporation, lipid interaction,
and binding to membrane components by these human milk folate binding
proteins will be studied in vitro. Finally, the role of these proteins in
absorption of folates in vivo will be tested in a rat model which will
closely simulates food folate absorption. These studies may allow for a
comprehensive understanding of the significance of folate binding proteins
of human milk and their role in the absorption of folates.
StatusFinished
Effective start/end date2/1/861/31/92

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Human Milk
Folic Acid
Carrier Proteins
Intestinal Secretions
Membranes
Microvilli
Glycosylation
Saliva
Intestines
Cultured Cells
Stomach
Milk
Proteins
Breast
Lipids
Food
Peptides

ASJC

  • Medicine(all)