FETAL BRANCHED-CHAIN AMINO ACID AND KETO-ACID METABOLISM

  • Liechty, Edward (PI)
  • dersimonian, R. (PI)

Project: Research project

Project Details

Description

The regulation of fetal protein synthesis and amino acid catabolism is
central to an understanding of fetal growth, normal or aberrant.
Investigations are best conducted with isotopic tracers, whereby the
dilution of the tracer is used as an estimate of production, utilization
and oxidation of the tracer amino acid. During the proposed project, work
will continue on the model of fetal leucine kinetics, utilizing infusion of
1-13C-15N-leucine into the fetal compartment and determination of leucine
carbon and nitrogen kinetics. Regulation of fetal leucine kinetics will be
explored during euglycemic and hyperglycemic clamp studies, while insulin
concentrations are maintained in the normal range, as well as during acute
hypo- and hyperinsulinemic euglycemia. Studies will also be begun to develop and validate a three compartment
fetal, uteroplacental and maternal model of leucine kinetics in the
pregnant ewe. While the isotopic modeling is complex, the analytical
methodology for this model is all presently being performed in our
laboratory. This model will be ultimately utilized to test hypotheses
regarding the manner in which alterations in maternal protein and nitrogen
kinetics affect fetal growth and nitrogen balance. Finally, I will be spending periods of time in the laboratories of
established experts in the fields of isotope modeling and mass
spectrometric analysis. At the present time, Drs. Robert Wolfe and John
Hayes have agreed to provide training and guidance in their respective
laboratories for periods of one to three months. I am confident these
sabbaticals will be invaluable in furthering my ability to design and apply
tracer methodology to problems of mammalian fetal growth.
StatusFinished
Effective start/end date12/1/8811/30/93

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $74,412.00
  • National Institutes of Health: $72,279.00

ASJC

  • Medicine(all)

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