CHARACTERIZATION OF PAPILLOMAVIRUS E6 PROTEINS

Project: Research project

Description

Bovine (B) papillomavirus (PV) type 1, which is genetically related
to human (H) and all other PVs, is the best studied model of PVs in
vitro. The BPV E6 gene fully transforms mouse cells and the
analogous E6 genes of HPVs are preferentially expressed in HPV
associated carcinomas. Utilizing antisera to bacterially expressed
BPV E6, this protein was identified in nuclear and membrane
fractions of BPV transformed cells. This represented the first
non-structural protein identified for any PV. The goals of this
proposal are to identify the corresponding E6 gene products of
HPVs and to biologically and functionally characterize the E6
proteins. The E6 gene of several HPVs, in particular those most
commonly found in human cancers, will be cloned into a bacterial
expression vector, antisera made and the gene products identified
and characterized via an analgous approach. Other studies
planned include the analysis of the metabolism and localization of
E6 in transformed cells and papillomas, and the testing for
biochemical activities such as binding to other viral or cellular
proteins or DNA. The biochemical properties of the BPV E6
protein will be tested in BPV E6 mutants and correlated with the
biological consequences of the mutation. To facilitate these
studies, a panel of monoclonal antibodies will be generated to the
BPV E6 protein. Monoclonals to HPV E6 proteins will also be
developed; in addition to their utility for experiments in this
protocol they may be of utility in the diagnosis and
characterization of PV containing dysplasias and carcinomas.
These studies should contribute to elucidating the mechanisms of
PV induced transformation and tumorigenesis.
StatusFinished
Effective start/end date7/1/874/30/96

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $286,144.00
  • National Institutes of Health
  • National Institutes of Health: $7,895.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Proteins
Neoplasms
Molecular Probes
Transcriptional Activation
Papillomavirus Infections
Viral Genes
Genes
Organized Financing
Oncogene Proteins
Oncogenes
Cervix Uteri
DNA
Saccharomyces cerevisiae
Immune Sera
Zinc
Transcription Factors
Bovine papillomavirus 1
Fibroblasts
Carcinoma
Papilloma

ASJC

  • Medicine(all)