EFFECTS OF CNS LESIONS ON MASTICATION AND SKULL FORM

Project: Research project

Description

The specific aims of this proposed research project are to test the effets
of unilateral trigeminal motor nucleus (TMNu) lesions upon (1) mandibular
movements during mastication, (2) muscle activity (EMG) patterns during
mastication, and (3) craniofacial form (muscle-bone alterations). Unilateral electrolytic lesions of the TMNu will be produced in actively
growing laboratory rats (Sprague-Dawley strain) at 40 days of age. After
sacrifice, muscles of mastication will be dissected and weighed to the
nearest 0.1 g in order to document the extent of postlesion muscle
atrophy. Both experimental and control rats will provide dry skull
preparations for osteometric analyses and brain histology preparations for
documentation of lesion extent. Before sacrifice, rats in each
experimental group will provide masticatory EMG and mandibular movement
data preoperative and at 4, 12, and 20 days postlesion; some rats will also
provide these data at 35, 55, 63, and 83 days postlesion. Frontal and
sagittal plane mandibular movement data will be obtained by the
optoelectronic method and simultaneously recorded with EMG data from left
and right side muscles of mastication. After digitizing and computer
analyses, these physiologic data will be correlated with postoperative
morphological data to assist interpretations of the lesion effects. Data from this proposed research project would be of value to clinicians
and basic researchers in dentistry, orthodontics, maxillofacial surgery,
neurophysiology, and functional anatomy. Such data will provide important
new detailed information on the actual role of the CNS and muscle function
in the development of craniofacial form. Since hemifacial microsomia,
human skull asymmetry, and associated TMJ dysfunctions are strongly
correlated with atrophy of muscles of mastication, detailed neuromuscular
physiologic data correlated with morphological changes will prove useful in
the establishment of future treatments for human craniofacial growth and
masticatory dysfunctions.
StatusFinished
Effective start/end date4/1/869/29/98

Funding

  • National Institutes of Health
  • National Institutes of Health: $100,523.00
  • National Institutes of Health: $95,276.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $77,921.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Mastication
Skull
Muscles
Neurotransmitter Agents
Glutamic Acid
Goldenhar Syndrome
Microspheres
Neurophysiology
Muscular Atrophy
Muscle Development
Oral Surgery
Temporomandibular Joint
Orthodontics
Dentistry
Research
Anatomy
Histology
Growth
Research Personnel
Growth Disorders

ASJC

  • Medicine(all)
  • Dentistry(all)