CELLULAR STUDIES OF BONE MARROW TRANSPLANTATION

  • Paczesny, Sophie (PI)
  • Cooke, Kenneth (PI)
  • Hanash, Samir (PI)
  • Nickoloff, Brian (PI)
  • Reddy, Pavan (PI)
  • Levine, John (PI)
  • Dranoff, Glenn (PI)
  • Kupper, Thomas S. (PI)
  • Antin, Joseph (PI)
  • Geha, Raif (PI)
  • Bierer, Barbara (PI)
  • Sykes, Megan (PI)
  • Nathan, David G. (PI)
  • Burakoff, Steven J. (PI)
  • Ferrara, James L. (PI)

Project: Research project

Description

Three major problems associated with bone marrow transplantation are: (1)
engraftment; (2) reconstitution of a normal immune system; and (3)
graft-versus-host disease (GVHD). Our program attempts to study these
problems at a molecular and cellular level. We have brought together
investigators with expertise in the areas of hematopoiesis, immunology,
molecular biology, and cell biology. Six projects in this proposed program
are directed at the above-mentioned issues and rely upon "reagents" from
the transplanted patients or their donors. A core section has been
requested to treat appropriate patients with T-cell-depleted bone marrow
transplants. The treated patients and their donors will provide reagents
required by the researchers in the six proposed projects. These reagents
will consist of blood, skin, fibroblast, and other tissues from the
transplant patients and their donors. The six projects are: (1) phenotypic characterization of returning cells
after bone marrow transplantation. Flow cytometry will be used to study
the cells of the immune system that repopulate the transplant patients; (2)
hematopoiesis following bone marrow transplantation will be studied in an
attempt to delineate the basis for the difficulty found for stem cell
engraftment, especially in haploidentical patients; (3) molecular biology
applications to the study of bone marrow transplantation. Molecular probes
will be used to study B-\and T-cell ontogeny, specifically when gene
rearrangement occurs in the immature T and B cells that repopulate the
transplant patient; (4) T-cell function after bone marrow transplantation.
Murine model systems will be utilized to study the problems of engraftment
and T-cell functions in the human bone marrow transplant patient will be
evaluated; (5) B-cell function after bone marrow transplantation. We will
attempt to determine whether the frequent B-cell aberrations observed in
these patients are due to dysfunctions in T-B interactions or whether the
dysfunction is inherent in the B cell itself; and (6) effector-target cell
interactions and GVHD in the skin. Skin histopathology will be studied as
a window on the tissue damage that occurs in GVHD. (TT)
StatusFinished
Effective start/end date9/30/856/30/16

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Proteomics
Quality Control
Databases
Research
Biomarkers
Technology

ASJC

  • Medicine(all)