CONTROL OF PAPILLOMAVIRUS EXPRESSION AND TRANSFORMATION

Project: Research project

Project Details

Description

DESCRIPTION The overall goal
of this research proposal is to examine in a comprehensive fashion, the
structural properties of the bovine papillomavirus (BPV) E2 protein that
mediate its transcription functions. The E2 gene product is central to
two pivotal events in the viral like cycle. E2 coordinates
transcription and is necessary for viral E1 protein. The 410 amino acid
E2 protein has distinct domains: it's amino-terminal third forms a dimer
that binds a specific papillomavirs DNA sequence with high affinity. The first specific aim of the proposal involves continued
characterization of the structure and function of the E2 protein's
carboxy terminus. The specific amino acids that mediate nucleic acid
recognition and dimerization as well as a newly-identified function
needed for transactivation represent the focus of attention. The second
specific aim has as its goal the genetic characterization of the
structure and functions of the E2 amino terminus in transactivation and,
to a limited extent viral DNA replication and E1 binding. Experiments
proposed in aim 3 use the results and resources provided by the
experiments in the first two aims to identify cellular proteins that bind
E2 and to determine how E2 functions are regulated in the cell. For
example, biochemical and mutational studies of the cysteine located in
the center of the DNA contact region and conserved among papillomavirus
E2 proteins suggest that its role may be to control E2 activity perhaps
through a nuclear localization pathway. The investigations proposed in
this application are designed to elucidate the molecular mechanisms
through which E2 stimulates transcription, binds DNA, and interacts with
cellular transcription factors and thereby provide insight into the
papillomavirus life cycle as well as into basic methods of controlling
gene expression.
StatusActive
Effective start/end date9/24/936/30/20

Funding

  • National Institutes of Health: $370,500.00
  • National Institutes of Health: $360,126.00
  • National Institutes of Health: $353,775.00
  • National Institutes of Health
  • National Institutes of Health: $9,510.00
  • National Institutes of Health: $283,213.00
  • National Institutes of Health: $232,326.00
  • National Institutes of Health: $341,289.00
  • National Institutes of Health: $312,214.00
  • National Institutes of Health: $360,126.00
  • National Institutes of Health: $360,126.00
  • National Institutes of Health
  • National Institutes of Health: $353,775.00
  • National Institutes of Health: $370,500.00
  • National Institutes of Health
  • National Institutes of Health: $345,461.00
  • National Institutes of Health: $393,776.00
  • National Institutes of Health: $351,551.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $266,999.00

ASJC

  • Medicine(all)

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