ANALYSIS OF A FRIEND VIRUS-LIKE DISEASE

  • Geib, Roy, (PI)

Project: Research project

Description

This project focuses on developing a better understanding of the
mechanisms involved in virus-induced leukemogenesis.
Specifically this project deals with host-controlled susceptibility
to a murine erythroleukemia virus. Fv-2 is a gene in the mouse
that modifies susceptibility to a single oncogenic virus, the Spleen
Focus-Forming Virus (SFFV). Mice homozygous for the Fv-2r
allele (e.g., C57BL/6) are completely resistant to transformation
by the spleen focus-forming virus (1). The experiments in this
project are devised to characterize the Friend virus-like disease
that develops in resistant mice infected with Fv-2rr-adapted
strains of Friend Virus. Experiments are proposed that will
examine several alternate theories on how the variants acquired
the new host range. The studies will examine the possibility that
the variants infect progenitor cells at a different stage of
differentiation than that infected by the wild-type virus. In
addition, the project proposes to clone the Fv-2rr-adapted viruses.
The cloned variants will then be analyzed by generating
recombinant with wild-type virus and by sequencing the env gene
of the variants. The results from this project will enhance our
understanding of viral-host interactions, virus-induced
transformation, and genetic control of erythroid differentiation.
StatusFinished
Effective start/end date7/1/886/30/94

Funding

  • National Institutes of Health: $57,886.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $62,352.00

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viruses
mice
oncogenic viruses
host range
stem cells
spleen
clones
genes

Keywords

  • Medicine(all)