FUNCTION OF NOVEL BOMBESIN BINDING PROTEINS FROM LUNG

  • Geraci, Mark, (PI)

Project: Research project

Description

The long term goal of this proposal is to elucidate the mechanism of
action of bombesin-like peptides (BLP) within the lungs by focusing on
the characterization of novel BLP binding proteins isolated from lung.
A growing body of evidence suggests that the BLP growth factor system
plays an important role in lung development, response to injury, and
carcinogenesis. As a major product of the pulmonary neuroendocrine cell,
BLP are mitogenic for bronchial epithelial cells and fibroblasts. They
appear to be involved in both lung growth and maturation. BLP have been
implicated to play a role in the pathogenesis of selected lung diseases
including: bronchopulmonary dysplasia, plexogenic pulmonary arteriopathy,
eosinophilic granuloma, and small cell lung cancer. Recently BLP
receptors have been cloned and appear to belong to a family of related
receptors of the G protein-coupled receptor superfamily. However, these
receptors have not been detected at the mRNA level in northern blots of
lung. My preliminary studies provide strong evidence for the existence of
previously undescribed BLP binding proteins in the lung. We hypothesize
that BLP effects within the lungs are modulated by these BLP binding
proteins, and that further characterization of the structure, expression,
and function of these BLP binding proteins may elucidate the role of BLP
in lung development and disease. We propose to examine the role of BLP
in the lung by molecular cloning of these novel BLP binding proteins
(Specific Aim 1), determining cell and tissue specific expression
(Specific Aim 2), and defining the function of these novel BLP binding
proteins (Specific Aim 3). These studies may be of therapeutic
importance by suggesting new approaches to either augmenting lung
development or interfering with the aberrant growth which occurs in
fibrotic diseases and cancer.
StatusFinished
Effective start/end date7/1/946/30/99

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Bombesin
Carrier Proteins
Lung
Peptides
Lung Diseases
Eosinophilic Granuloma
Bronchopulmonary Dysplasia
Neuroendocrine Cells
Small Cell Lung Carcinoma
Molecular Cloning
Growth
G-Protein-Coupled Receptors
Northern Blotting
Intercellular Signaling Peptides and Proteins

Keywords

  • Medicine(all)