MOLECULAR AND CELLULAR BIOLOGY OF HUMAN GLIAL TUMORS

  • Dropcho, Edward (PI)
  • Gillespie, Yancey (PI)
  • Benveniste, Etty (PI)
  • Gladson, Candece (PI)
  • Rosenfeld, Steven (PI)

Project: Research project

Description

The central goal of this project is to establish a center for basic and
clinical brain tumor research. Specific emphasis has been placed on
studies of the molecular and cellular biology of anaplastic glial tumors,
and on investigational treatment programs for patients affected by these
tumors. Project No.1 is concerned with expression of tumor necrosis
factor-alpha (TNFalpha) in malignant glioma cells. Studies will focus on
induction of TNFalpha gene expression by interleukin-1, on transcriptional
control elements involved in TNFalpha expression, and on the effects of
TNFalpha on glioma cell biology. Project No.2 will examine the role of
transforming growth factor-beta (TGFbeta) in the cellular biology of glioma
cells. Studies will focus on purifying, cloning, and characterizing a
novel high-molecular weight form of TGFbeta expressed by human gliomas and
glioma cell lines. Project No.3 is concerned with characterizing the
expression of vitronectin and of the vitronectin receptor integrins by
glioma cells in situ and in vitro. The functional role of these integrins
in adhesion and invasiveness of glioma cells will be studied, as will the
effects on glioma cells of blocking integrin function. Project No.4 will
examine the molecular basis for the motility and invasiveness of glioma
cells. Studies will focus on the functional topology of glioma-derived
myosin II, and on the inhibitors of myosin II and integrin function as
potential antagonists of glioma cell invasiveness. Project No.5 will
continue the active clinical efforts at UAB to develop better therapy for
patients with malignant gliomas. Treatment programs will include intra-
arterial cisplatin chemotherapy, intensive systemic chemotherapy, biologic
response modifiers, and studies of the safety and efficacy of an anti-
epidermal growth factor receptor monoclonal antibody. In addition, the
practical clinical usefulness of single photon emission computed tomography
(SPECT) imaging of malignant gliomas and the biologic insights provided by
this technique will be studied. Core A, the Administrative Core, will
facilitate interactions among the group members, and will be responsible
for the overall management of the project.
StatusFinished
Effective start/end date9/30/927/31/96

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

Fingerprint

Glioma
Neuroglia
Cell Biology
Molecular Biology
Clinical Trials
Neoplasms
Integrins
Drug Therapy
Monoclonal Antibodies
Cisplatin
Myosin Type II
Transforming Growth Factor beta
Vitronectin
Thallium
Clinical Protocols
Safety
Single-Photon Emission-Computed Tomography
Radiotherapy
Tumor Necrosis Factor-alpha
Intercellular Signaling Peptides and Proteins

ASJC

  • Medicine(all)
  • Neuroscience(all)