CHARACTERIZATION OF A >400,000-DALTON CARTILAGE PROTEIN

Project: Research project

Description

Various non-collagenous, non-proteoglycan proteins have been described in
articular and non-articular cartilage. The function of most of these
proteins is unknown. We have recently identified a high molecular weight
(greater than 400,000-dalton) protein, with 116,000-dalton subunits, in all
normal articular cartilage studied, as well as in normal bovine tracheal
cartilage, atrophic canine knee cartilage, and osteoarthritic human hip
cartilage. This protein constitutes approximately 3-7% of the total
non-collagenous protein in 4 M guanidinium chloride extracts of normal
articular cartilage and is one of the major non-collagenous proteins
synthesized in 24-hours organ cultures of normal canine knee cartilage.
Immunofluorescence studies using specific antiserum reveal that the
116,000-dalton protein is found in the interterritorial and pericellular
regions of normal adult and fetal cartilage. Only a small amount of
cross-reacting material is present in menisci, synovium, and synovial cell
cultures. This protein is not present in serum or synovial fluid, which
provides evidence that it is not adsorbed from these sources onto cartilage. The goals of the studies proposed in this application are to purify the
greater than 400,000-dalton protein from articular cartilage of immature,
mature, and osteoarthritic dogs; to explore its interactions with collagen,
proteoglycans, and hyaluronic acid; to quantitate it in the various
cartilages; and to study its biochemical composition and metabolism. This
project will thus contribute new information concerning what appears to be
a major non-collagenous protein of normal articular cartilage. It may also
enlarge our understanding of the macromolecular composition of cartilage in
aging and ostearthritis.
StatusFinished
Effective start/end date9/20/858/31/88

Funding

  • National Institutes of Health

Fingerprint

Cartilage
Articular Cartilage
Proteins
Canidae
Knee
Synovial Membrane
Organ Culture Techniques
Synovial Fluid
Guanidine
Proteoglycans
Hyaluronic Acid
Fluorescent Antibody Technique
Immune Sera
Collagen
Molecular Weight
Dogs
Serum

ASJC

  • Medicine(all)