GLYCOGEN METABOLISM AND SIGNAL TRANSDUCTION IN YEAST

Project: Research project

Description

The broad objective of this project is to understand the molecular
mechanisms by which extracellular signals regulate the intracellular
events. Glycogen metabolism, has long been a model for studying such
controls which, in mammals, involve hormones such as insulin, glucagon
and catecholamines. This project focuses on signalling pathways in the
yeast Saccharomyces cerevisiae involved in regulating glycogen
metabolism, and especially the enzyme glycogen synthase. In preliminary
work, yeast glycogen synthase has been purified and one corresponding
gene cloned. The specific aims are: (i) to complete characterization of
the yeast enzyme and establish how many glycogen synthase genes are
present in yeast. The purified protein contains two related
polypeptides, 77 and 85 Kda, and their exact relationship to each other
and the glycogen synthase genes (if more than one) is still unknown.;
(ii) to identify which sites in the enzyme are phosphorylated in whole
cells and to define the corresponding protein kinase(s); (iii) to
correlate in vitro phosphorylation of individual sites with changes in
enzymic properties; and (iv) to analyze changes in glycogen synthase
phosphorylation in response to different physiological situations,
incorporating mutants affecting the signalling pathways and mutations,
introduced by reverse genetics, in the glycogen synthase itself. The
goal is a better molecular understanding of protein phosphorylation and
signalling mechanisms in yeast. In turn, such results should provide a
broader perspective on intracellular signalling that can be relevant to
understanding similar processes in mammals, including control by
hormones. The research, though fundamental in nature, is relevant to
situations of impaired hormonal control, such as in diabetes.
StatusFinished
Effective start/end date1/4/9111/30/07

Funding

  • National Institutes of Health
  • National Institutes of Health: $285,879.00
  • National Institutes of Health
  • National Institutes of Health: $215,747.00
  • National Institutes of Health: $295,893.00
  • National Institutes of Health: $294,455.00
  • National Institutes of Health: $147,485.00
  • National Institutes of Health
  • National Institutes of Health: $295,830.00
  • National Institutes of Health: $295,954.00
  • National Institutes of Health
  • National Institutes of Health: $174,867.00
  • National Institutes of Health
  • National Institutes of Health: $203,882.00

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glycogen (starch) synthase
Glycogen
Signal Transduction
Yeasts
glycogen
signal transduction
yeasts
metabolism
Glycogen Synthase
Autophagy
Glycogen Synthase Kinases
enzymes
Cyclin-Dependent Kinases
mammals
Protein Kinases
Genes
protein phosphorylation
hormonal regulation
Glucose
catecholamines

ASJC

  • Medicine(all)