TRANSCRIPTION FACTORS IN KIDNEY DEVELOPMENT

Project: Research project

Description

In mammalian embryos, the metanephric kidney emerges from metanephric
mesenchyme and ureteric bud epithelium, a process characterized by the
synthesis of numerous new epithelial proteins and suppression of the
activity of mesenchymal genes. I propose to study some of the many
transcription factors which must be involved in directing this new gene
actiVity. I have begun to approach this investigation by performing
differential hybridization to find genes expressed in embryonic but not
adult kidney and by screening an embryonic kidney cDNA library with probes
for transcription factor motifs. One or two developmentally regulated
candidate transcription factors will be studied by overexpression in
cultured cells, by analyzing the promoter activity using luciferase
reporter gene constructs, by investigation of transcriptional modulation
through the use of chimeric transcription factor constructs, and
identification of genes that are regulated by one of these transcription
factor proteins. The study of transcription factors important in normal
development of the kidney will improve our understanding of normal and
disordered kidney growth and differentiation as well as neoplasia, and
possibly recovery from acute renal injury. Knowledge gained from this work
may help in the development of new therapies for acute renal failure and
congenital kidney diseases.
StatusFinished
Effective start/end date8/1/947/31/00

Funding

  • National Institutes of Health: $96,234.00
  • National Institutes of Health: $5,917.00
  • National Institutes of Health: $87,228.00
  • National Institutes of Health
  • National Institutes of Health: $115,739.00
  • National Institutes of Health

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Transcription Factors
Kidney
Acute Kidney Injury
Genes
Mammalian Embryo
Kidney Diseases
Gene Library
Proteins
Epithelium
Growth
Mesoderm
Luciferases
Reporter Genes
Neoplasms
Cultured Cells
Therapeutics

ASJC

  • Medicine(all)