DEVELOPING QUANTITATIVE-INTRAVITAL AND 3D MICROSCOPY OF THE KIDNEY

Project: Research project

Description

Description: (Taken directly from the application)
Newly developed technologies in microscopy and digital image analysis have
given researchers the potential to characterize kidney tissues in ways not
previously possible. Over the past 5 years we have actively applied these
technologies to develop new imaging approaches to the study of renal biology,
including 3-dimensional microscopy, quantitative microscopy and high-resolution
imaging of cells in living animals. However, we have found that effective
implementation of each of these powerful techniques is limited by the lack of
fundamental information about multi-photon microscopy, deep-tissue microscopy
and 3-dimensional image analysis. It is the objective of this project to
develop an integrated approach of 3-dimensional microscopy and image analyses
to support renal research. Specifically, we propose to, 1. Develop and optimize
methods for collection of microscopic images of renal tissues. 2. Develop
quantitative methods for microscopic analysis of tissues in, living animals. 3.
Develop methods for 3-dimensional image analysis. The unique combination of
state-of-the-art resources and expertise of the IU nephrology imaging facility
represents a unique opportunity to develop a center of excellence in
3-dimensional microscopy in renal research. Such a center addresses a need that
has otherwise been ignored in biomedical research. Furthermore, the development
of this center will provide renal researchers with a novel set of research
tools not available elsewhere, tools that will facilitate biomedical research,
drug discovery, and solutions to human health problems.
StatusFinished
Effective start/end date4/1/025/31/07

Funding

  • National Institutes of Health
  • National Institutes of Health: $1,034,256.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $34,642.00
  • National Institutes of Health: $1,029,813.00
  • National Institutes of Health: $1,042,006.00
  • National Institutes of Health: $1,036,472.00
  • National Institutes of Health: $1,033,698.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $34,536.00
  • National Institutes of Health: $37,634.00

Fingerprint

Kidney
Research Personnel
Technology
Photons
Nephrology
Research
Cultured Cells
Lasers
Software
Light
Microscopy
Therapeutics
Acidosis
Pyruvic Acid
Acute Kidney Injury
Oxidoreductases
Reperfusion Injury
Endothelial Cells
Apoptosis
Wounds and Injuries

ASJC

  • Medicine(all)