PEPTIDE GROWTH FACTORS AND SKELETAL DEVELOPMENT

  • Trippel, Stephen, (PI)

Project: Research project

Description

DESCRIPTION: Virtually all
skeletal growth is the result of the orderly division and maturation of
the cells of the epiphyseal growth plate. Normal skeletal development
is dependent on the proper control of this process. This proposal is
based on the applicants' previous observations that peptide growth
factors participate in this control. The goal of the proposed studies
is to elucidate the mechanisms by which selected growth factors regulate
growth plate chondrocytes. The investigators hypothesize that growth
factor interaction plays an important role in the regulation of growth
plate chondrocytes. One aim of these studies is to test this hypothesis
by examining the individual and interactive effects of selected growth
factors with respect to mitotic and differentiated chondrocyte functions
in vitro. Cell culture, metabolic labelling, radioimmunoassay,
immunoinhibition, Northern and in situ hybridization methods will be used
to identify interactions and begin to elucidate their mechanisms. The
second aim is to test the postulate that the effects of the calciotropic
hormones, calcitonin and PTH, on growth plate chondrocytes are modulated
by peptide growth factors. Specifically, regulation of chondrocyte PTH
receptor and calcitonin receptor expression by selected growth factors
will be examined by cell biological and molecular biological techniques.
The third aim is to assess the ability of exogenously administered
growth factors to regulate skeletal growth in vivo.Using a rat model,
the action and interaction of selected factors will be evaluated by
metabolic and in situ hybridization techniques. The long-term objective
of these studies is to help achieve a basic understanding of the
mechanisms regulating skeletal growth. Only with such information can
abnormalities of skeletal growth and repair be understood and the
potential contribution of growth factors to clinical problems eventually
be determined.
StatusFinished
Effective start/end date9/1/818/31/01

Funding

  • National Institutes of Health
  • National Institutes of Health: $192,475.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $209,995.00
  • National Institutes of Health: $192,056.00
  • National Institutes of Health
  • National Institutes of Health: $215,313.00

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Growth Plate
Intercellular Signaling Peptides and Proteins
Chondrocytes
Peptides
Insulin-Like Growth Factor I
Growth
Somatomedins
Growth Hormone
In Situ Hybridization
Calcitonin Receptors
Histological Techniques
Calcitonin
Growth and Development
Radioimmunoassay
Pathology
Cell Culture Techniques
Research Personnel

ASJC

  • Medicine(all)