MECHANISMS OF HEMOPOIETIC IMMORTALIZATION

Project: Research project

Project Details

Description

The goals of the proposed work are: 1) development of
interleukin-3 (IL-3) dependent cell lines from normal mouse
marrow cells to be used to study the molecular events occurring
during IL-3 dependent cellular proliferation; 2) comparison of the
effect of hemopoietic growth factors on the factor-dependent cell
lines and purified populations of hemopoietic progenitor cells.
These studies will concentrate on post-ligand receptor events that
occur in response to growth factors or hormones that induce
differentiation; 3) correlation between cellular proto-oncogene
expression, production of second messengers, and responsiveness
to leukemic cell inducing agents of factor-dependent cell lines; 4)
examination of the responsiveness of immortal factor-dependent
and factor-independent cell lines to prostaglandin E and the role
of the GTP-binding protein that stimulates adenylate cyclase (Gs)
in this phenomenon; and 5) murine myeloid leukemic cell lines
whose proliferation is dependent on added hemopoietic growth
factors will be transfected with cloned genes for IL-3 or GM-CSF.
The mode of expression of the newly transfected genes will be
studied to further define the potential role of autocrine
regulatory mechanisms in leukemogenesis.
StatusFinished
Effective start/end date6/15/875/31/92

Funding

  • National Institutes of Health: $113,283.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)

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