α Adrenergic reduction of cyclic adenosine monophosphate concentrations in rat myocardium

A. M. Watanabe, D. R. Hathaway, H. R. Besch, B. B. Farmer, Robert Harris

Research output: Contribution to journalArticle

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Abstract

The authors determined the effect of α-adrenergic receptor stimulation on cyclic adenosine monophosphate (cyclic AMP) concentrations in isolated myocytes derived from adult rat hearts and in isolated perfused rat hearts. Activation of α adrenergic receptors with either phenylephrine (10 -8M to 10 -6M) or epinephrine (10 -8M to 10 -6M) plus propranolol (10 -6M) resulted in a reduction in cyclic AMP levels in isolated myocytes. The action of phenylephrine was antagonized by phentolamine (10 -6M). Phenylephrine (10 -5M) attenuated cyclic AMP generation in response to isoproterenol (10 -8M and 10 -5M). However, this effect of phenylephrine was not antagonized by phentolamine. Elevation of cyclic AMP concentrations produced by glucagon and by theophylline in isolated mycocytes was attenuated by phenylephrine and by epinephrine plus propranolol and the attenuation was antagonized by phentolamine. In isolated perfused rat hearts epinephrine (10 -6M), when given with propranolol, diminished the rate of development of tension and also reduced tissue levels of cyclic AMP. Epinephrine alone, as well as isoproterenol, increased contractility and myocardial cyclic AMP concentrations as expected. These results indicate that catecholamines may increase or decrease cyclic AMP levels in rat myocardium, depending on the intensity of stimulation of receptor types. Increases are mediated by β adrenergic receptors, whereas decreases appear to be mediated by α-adrenergic receptors.

Original languageEnglish (US)
Pages (from-to)596-602
Number of pages7
JournalCirculation Research
Volume40
Issue number6
StatePublished - Dec 1 1977
Externally publishedYes

Fingerprint

Cyclic AMP
Adrenergic Agents
Myocardium
Phenylephrine
Adrenergic Receptors
Epinephrine
Phentolamine
Propranolol
Isoproterenol
Muscle Cells
Theophylline
Glucagon
Catecholamines

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Watanabe, A. M., Hathaway, D. R., Besch, H. R., Farmer, B. B., & Harris, R. (1977). α Adrenergic reduction of cyclic adenosine monophosphate concentrations in rat myocardium. Circulation Research, 40(6), 596-602.

α Adrenergic reduction of cyclic adenosine monophosphate concentrations in rat myocardium. / Watanabe, A. M.; Hathaway, D. R.; Besch, H. R.; Farmer, B. B.; Harris, Robert.

In: Circulation Research, Vol. 40, No. 6, 01.12.1977, p. 596-602.

Research output: Contribution to journalArticle

Watanabe, AM, Hathaway, DR, Besch, HR, Farmer, BB & Harris, R 1977, 'α Adrenergic reduction of cyclic adenosine monophosphate concentrations in rat myocardium', Circulation Research, vol. 40, no. 6, pp. 596-602.
Watanabe, A. M. ; Hathaway, D. R. ; Besch, H. R. ; Farmer, B. B. ; Harris, Robert. / α Adrenergic reduction of cyclic adenosine monophosphate concentrations in rat myocardium. In: Circulation Research. 1977 ; Vol. 40, No. 6. pp. 596-602.
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