α5β1 integrin as a cellular coreceptor for human parvovirus B19: Requirement of functional activation of β1 integrin for viral entry

Kirsten A. Weigel-Kelley, Mervin Yoder, Arun Srivastava

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

Replication of the pathogenic human parvovirus B19 is restricted to erythroid progenitor cells. Although blood group P antigen has been reported to be the cell surface receptor for parvovirus B19, a number of nonerythroid cells, which express P antigen, are not permissive for parvovirus B19 infection. We have documented that P antigen is necessary for parvovirus B19 binding but not sufficient for virus entry into cells. To test whether parvovirus B19 utilizes a cell surface coreceptor for entry, we used human erythroleukemia cells (K562), which allow parvovirus B19 binding but not entry. We report here that upon treatment with phorbol esters, K562 cells become adherent and permissive for parvovirus B19 entry, which is mediated by α5β1 integrins, but only in their high-affinity conformation. Mature human red blood cells (RBCs), which express high levels of P antigen, but not α5β1 integrins, bind parvovirus B19 but do not allow viral entry. In contrast, primary human erythroid progenitor cells express high levels of both P antigen and α5β1 integrins and allow β1 integrin-mediated entry of parvovirus B19. Thus, in a natural course of infection, RBCs are likely exploited for a highly efficient systemic dissemination of parvovirus B19.

Original languageEnglish (US)
Pages (from-to)3927-3933
Number of pages7
JournalBlood
Volume102
Issue number12
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

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Human Parvovirus B19
Parvovirus
Integrins
Chemical activation
Antigens
Blood
Erythroid Precursor Cells
K562 Cells
Cell Surface Receptors
Phorbol Esters
Blood Group Antigens
Viruses
Erythrocytes
Conformations
Parvoviridae Infections
Virus Internalization
Leukemia, Erythroblastic, Acute
Cells
Cell Count

ASJC Scopus subject areas

  • Hematology

Cite this

α5β1 integrin as a cellular coreceptor for human parvovirus B19 : Requirement of functional activation of β1 integrin for viral entry. / Weigel-Kelley, Kirsten A.; Yoder, Mervin; Srivastava, Arun.

In: Blood, Vol. 102, No. 12, 01.12.2003, p. 3927-3933.

Research output: Contribution to journalArticle

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