β-Adrenergic stimulation of aldosterone production by rat adrenal capsular explants

J. H. Pratt, D. A. Turner, J. A. McAteer, D. P. Henry

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Adrenal glomerulosa was examined for the presence of an adrenergic influence on aldosterone production. Cultured rat adrenal capsular explants were transferred to a perifusion system where the effect of exposure to catecholamines on aldosterone production was assessed. At 10-6 M, isoproterenol greater than epinephrine greater than norepinephrine significantly stimulated aldosterone production, whereas at 10-8 M only isoproterenol showed significant stimulation. Propranolol, a β-adrenoreceptor antagonist, inhibited stimulation by epinephrine, and the phosphodiesterase inhibitor, 1-methyl-3-isobutylxanthine, enhanced stimulation by a submaximal dose of epinephrine. Epinephrine and norepinephrine were found by radioenzymatic assay to be present in fresh as well as cultured capsular tissue, although levels were considerably lower in tissue that had been in culture (about one tenth that of fresh tissue). The epinephrine-norepinephrine ratio was similar in capsule and medulla, suggesting a medullary source of capsular catecholamines. Whether catecholamines in the capsule arose from the in vitro manipulation of adrenal tissue or existed in vivo is unclear. In summary, β-agonists stimulate aldosterone production in cultured rat capsular explants.

Original languageEnglish
Pages (from-to)1189-1194
Number of pages6
JournalEndocrinology
Volume117
Issue number3
StatePublished - 1985
Externally publishedYes

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Aldosterone
Adrenergic Agents
Epinephrine
Catecholamines
Norepinephrine
Isoproterenol
Capsules
Zona Glomerulosa
1-Methyl-3-isobutylxanthine
Phosphodiesterase Inhibitors
Propranolol

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Pratt, J. H., Turner, D. A., McAteer, J. A., & Henry, D. P. (1985). β-Adrenergic stimulation of aldosterone production by rat adrenal capsular explants. Endocrinology, 117(3), 1189-1194.

β-Adrenergic stimulation of aldosterone production by rat adrenal capsular explants. / Pratt, J. H.; Turner, D. A.; McAteer, J. A.; Henry, D. P.

In: Endocrinology, Vol. 117, No. 3, 1985, p. 1189-1194.

Research output: Contribution to journalArticle

Pratt, JH, Turner, DA, McAteer, JA & Henry, DP 1985, 'β-Adrenergic stimulation of aldosterone production by rat adrenal capsular explants', Endocrinology, vol. 117, no. 3, pp. 1189-1194.
Pratt JH, Turner DA, McAteer JA, Henry DP. β-Adrenergic stimulation of aldosterone production by rat adrenal capsular explants. Endocrinology. 1985;117(3):1189-1194.
Pratt, J. H. ; Turner, D. A. ; McAteer, J. A. ; Henry, D. P. / β-Adrenergic stimulation of aldosterone production by rat adrenal capsular explants. In: Endocrinology. 1985 ; Vol. 117, No. 3. pp. 1189-1194.
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