β-Catenin control of T-cell transcription factor 4 (Tcf4) importation from the cytoplasm to the nucleus contributes to Tcf4-mediated transcription in 293 cells

Hui Ting Hsu, Po Chun Liu, Sheng Yu Ku, Kuo Chen Jung, Yi Ren Hong, Chinghai Kao, Chihuei Wang

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

β-Catenin has essential roles in morphogenesis and human cancer, both as a subunit of adhesive complexes in the cell membrane and as a transcriptional coactivator in the Wnt signaling pathway. In addition, β-catenin also has the ability to transport lymphoid enhancer binding factor-1 into the nucleus. In this study, we examined a constitutive active mutation, β-catenin (T41A, S45A), for its potential as a nuclear import receptor for T-cell transcription factor 4 in 293 cells. Immunoblot analysis demonstrated that this constitutive active form of β-catenin increased the amount of Tcf4 in the nucleus about 4-5-fold compared to controls. However, the overall expression of Tcf4 remained the same with or without over-expression of β-catenin (T41A, S45A). T-cell transcription factor 4 reporter gene and electrophoretic mobility shift assay further indicated that the increase in Tcf4 in the nucleus was consistent with its accrued DNA binding capacity and transcription activity. Microscopic immunofluorescence examination showed that Tcf4 was mainly located in the cytoplasm and transported into the nucleus, without or with over-expression of β-catenin (T41A, S45A), respectively. Our results suggest that β-catenin might be a major factor regulating the import of Tcf4 from the cytoplasm into the nucleus, consequently controlling its transcription activity.

Original languageEnglish (US)
Pages (from-to)893-898
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume343
Issue number3
DOIs
StatePublished - May 12 2006

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Keywords

  • β-Catenin
  • Coactivator
  • Lymphoid enhancer binding factor-1
  • Nuclear import receptor
  • T-cell transcription factor 4
  • Transcription
  • Wnt signaling pathway

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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