The diversity of human T cell receptor β-chain gene rearrangements and variable region gene usage in the T cell response to a single allogeneic class II HLA gene product has been investigated. Nine clones of cytotoxic T lymphocytes (8.2 to 8.10) that are specific for the class II specificity DPw2 were analyzed for their T cell receptor β-chain gene rearrangements using a constant-region probe. A minimum of seven different clonotypes were present in this panel of clones. The β-gene expressed by one clone, 8.9, was isolated and the variable (V), diversity (D), and joining (J) segments were sequenced. The sequence of the Vβ 8.9 segment is identical to the Vβ 14 sequence, and is joined to Dβ 1.1 and Jβ 1.1 segments. Northern analysis revealed that three of the eight clones analyzed, 8.5, 8.7, and 8.9, expressed a 1.3 kb transcript that hybridized with the Vβ 8.9 probe, indicating that these clones were using the same Vβ gene (these clones shared common DNA rearrangements). The remaining five cloned did not express Vβ 8.9. Southern analysis of DNA obtained from a DPw2-specific tertiary mixed lymphocyte reaction bulk culture from which the clones were derived showed a prominent rearrangement of the Vβ 8.9 gene that was indistinguishable from those observed for clones 8.5, 8.7, and 8.9. This prominent rearrangement of Vβ 8.9 was not observed in DNA obtained from normal peripheral blood lymphocytes. These results suggest that although the number of Vβ genes which can contribute to a DPw2 specificity may be relatively large, only a limited number of clonotypes ultimately predominate in the response to certain class II HLA antigens.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Immunology|
|State||Published - Nov 9 1987|
ASJC Scopus subject areas
- Immunology and Allergy