β-Galactosidase detection as an indicator of endogenous PTHrP in cartilage

X. S. Chen, N. W. Troiano, M. A. Kacena

Research output: Contribution to journalArticle

Abstract

Genetically altered mice are an important tool for biomedical research. Several transgenic mice have been created in which activation of the transgene results in production of β-galactosidase that can be detected by histological means. While preservation and subsequent visualization of enzyme activity in soft tissues can be complicated, it is particularly difficult in bone specimens, especially those that have been decalcified. For these studies, we examined the bones of parathyroid hormone-related peptide (PTHrP) knock-in mice in which expression of PTHrP resulted in β-galactosidase production. During the past decade, several studies have demonstrated the importance of PTHrP in bone. Thus, it is important to preserve and detect β-galactosidase enzymatic activity in bone for these studies. We demonstrate here that β-galactosidase was visualized better in slides with bone sections taken from PTHrP knock-in mice when bones were frozen and sectioned compared to bones that were embedded in plastic and sectioned using a microtome. Importantly, we were able to visualize β-galactosidase in plastic embedded bones when specimens were fixed, stained (X-gal), embedded in plastic, and then sectioned rather than being fixed, embedded in plastic, sectioned, then stained.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalBiotechnic and Histochemistry
Volume83
Issue number2
DOIs
StatePublished - Mar 1 2008

Keywords

  • Enzyme preservation
  • GMA
  • Histology
  • Methacrylate
  • MMA
  • Parathyroid hormone-related peptide
  • PTHrP
  • X-gal staining

ASJC Scopus subject areas

  • Anatomy
  • Applied Microbiology and Biotechnology

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