β2-Microglobulin induces calcium efflux from cultured neonatal mouse calvariae

Sharon Moe, Stuart M. Sprague

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

β2-Microglobulin (β2M) polymerizes to form amyloid fibrils that deposit and cause destructive bone lesions in patients on chronic dialytic therapy. β2M is mitogenic to osteoblasts; however, its effect on bone mineralization is unknown. To determine whether β2M causes bone demineralization, neonatal mouse calvariae were incubated with and without β2M, and net calcium flux was calculated. Following a 48-h but not 3- or 24-h incubation, β2M (10-8-10-6 M) induced a net calcium efflux. The efflux was similar to that observed with 10-10 M parathyroid hormone (PTH) but less than that observed with 10-8 M PTH. Devitalizing the calvariae resulted in a net calcium influx that was unaffected by the addition of β2M, indicating a cell-mediated phenomenon. The release of β-glucuronidase, an osteoclast enzyme, increased after a 48-h but not a 24-h incubation with β2M. Calcitonin, an osteoclast inhibitor, blocked the β2M-induced calcium efflux and β-glucuronidase release, suggesting osteoclast involvement. Thus β2M induces a dose- and time-dependent, cell-mediated calcium efflux from neonatal mouse calvariae that involves osteoclast stimulation.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume263
Issue number3 32-3
StatePublished - Sep 1992

Fingerprint

Skull
Osteoclasts
Calcium
Glucuronidase
Parathyroid Hormone
Bone and Bones
Physiologic Calcification
Amyloid Plaques
Calcitonin
Osteoblasts
Amyloid
Enzymes
Therapeutics

Keywords

  • β-microglobulin amyloidosis
  • Bone resorption
  • Osteoblast
  • Osteoclast

ASJC Scopus subject areas

  • Physiology

Cite this

β2-Microglobulin induces calcium efflux from cultured neonatal mouse calvariae. / Moe, Sharon; Sprague, Stuart M.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 263, No. 3 32-3, 09.1992.

Research output: Contribution to journalArticle

@article{4ba1980ab03a445e88cf7f3131d4b1e5,
title = "β2-Microglobulin induces calcium efflux from cultured neonatal mouse calvariae",
abstract = "β2-Microglobulin (β2M) polymerizes to form amyloid fibrils that deposit and cause destructive bone lesions in patients on chronic dialytic therapy. β2M is mitogenic to osteoblasts; however, its effect on bone mineralization is unknown. To determine whether β2M causes bone demineralization, neonatal mouse calvariae were incubated with and without β2M, and net calcium flux was calculated. Following a 48-h but not 3- or 24-h incubation, β2M (10-8-10-6 M) induced a net calcium efflux. The efflux was similar to that observed with 10-10 M parathyroid hormone (PTH) but less than that observed with 10-8 M PTH. Devitalizing the calvariae resulted in a net calcium influx that was unaffected by the addition of β2M, indicating a cell-mediated phenomenon. The release of β-glucuronidase, an osteoclast enzyme, increased after a 48-h but not a 24-h incubation with β2M. Calcitonin, an osteoclast inhibitor, blocked the β2M-induced calcium efflux and β-glucuronidase release, suggesting osteoclast involvement. Thus β2M induces a dose- and time-dependent, cell-mediated calcium efflux from neonatal mouse calvariae that involves osteoclast stimulation.",
keywords = "β-microglobulin amyloidosis, Bone resorption, Osteoblast, Osteoclast",
author = "Sharon Moe and Sprague, {Stuart M.}",
year = "1992",
month = "9",
language = "English",
volume = "263",
journal = "American Journal of Physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "3 32-3",

}

TY - JOUR

T1 - β2-Microglobulin induces calcium efflux from cultured neonatal mouse calvariae

AU - Moe, Sharon

AU - Sprague, Stuart M.

PY - 1992/9

Y1 - 1992/9

N2 - β2-Microglobulin (β2M) polymerizes to form amyloid fibrils that deposit and cause destructive bone lesions in patients on chronic dialytic therapy. β2M is mitogenic to osteoblasts; however, its effect on bone mineralization is unknown. To determine whether β2M causes bone demineralization, neonatal mouse calvariae were incubated with and without β2M, and net calcium flux was calculated. Following a 48-h but not 3- or 24-h incubation, β2M (10-8-10-6 M) induced a net calcium efflux. The efflux was similar to that observed with 10-10 M parathyroid hormone (PTH) but less than that observed with 10-8 M PTH. Devitalizing the calvariae resulted in a net calcium influx that was unaffected by the addition of β2M, indicating a cell-mediated phenomenon. The release of β-glucuronidase, an osteoclast enzyme, increased after a 48-h but not a 24-h incubation with β2M. Calcitonin, an osteoclast inhibitor, blocked the β2M-induced calcium efflux and β-glucuronidase release, suggesting osteoclast involvement. Thus β2M induces a dose- and time-dependent, cell-mediated calcium efflux from neonatal mouse calvariae that involves osteoclast stimulation.

AB - β2-Microglobulin (β2M) polymerizes to form amyloid fibrils that deposit and cause destructive bone lesions in patients on chronic dialytic therapy. β2M is mitogenic to osteoblasts; however, its effect on bone mineralization is unknown. To determine whether β2M causes bone demineralization, neonatal mouse calvariae were incubated with and without β2M, and net calcium flux was calculated. Following a 48-h but not 3- or 24-h incubation, β2M (10-8-10-6 M) induced a net calcium efflux. The efflux was similar to that observed with 10-10 M parathyroid hormone (PTH) but less than that observed with 10-8 M PTH. Devitalizing the calvariae resulted in a net calcium influx that was unaffected by the addition of β2M, indicating a cell-mediated phenomenon. The release of β-glucuronidase, an osteoclast enzyme, increased after a 48-h but not a 24-h incubation with β2M. Calcitonin, an osteoclast inhibitor, blocked the β2M-induced calcium efflux and β-glucuronidase release, suggesting osteoclast involvement. Thus β2M induces a dose- and time-dependent, cell-mediated calcium efflux from neonatal mouse calvariae that involves osteoclast stimulation.

KW - β-microglobulin amyloidosis

KW - Bone resorption

KW - Osteoblast

KW - Osteoclast

UR - http://www.scopus.com/inward/record.url?scp=0026775905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026775905&partnerID=8YFLogxK

M3 - Article

C2 - 1415583

AN - SCOPUS:0026775905

VL - 263

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1857

IS - 3 32-3

ER -