1α,25-dihydroxyvitamin D3 and analogues of vitamin D3 induce alkaline phosphatase activity in osteoblastic cells derived from newborn mouse calvaria

Tatsuji Haneji, Noriyoshi Kurihara, Katsumi Ikeda, Masayoshi Kumegawa

Research output: Contribution to journalArticle

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Abstract

In order to study the effects of vitamin D metabolites on bone metabolism, clone MC3T3-E1 cells, which have retained osteoblastic activity, were cultured with various concentrations of the hormone, 1α,25-dihydroxyvitamin D3 [1α,25 (OH)2D3]. A physiological concentration of 1α,25 (OH)2D3 stimulated alkaline phosphatase (ALP) activity in the cells. Other metabolites-1α,24-dihydroxyvitamin D3 [1α,24 (OH)2D3], 1α-hydroxyvitamin D3 [1α (OH)D3], and 24R,25-dihydroxyvitamin D3 [24R,25 (OH)2D3]-also induced increases in ALP activity in a dose-dependent fashion. However, their effective concentrations were 100 or 1,000 times greater than that of 1α,25 (OH)2D3. Hormone-induced and native ALP activities in the cells were of the same type as that found in newborn mouse calvaria; that is, they were heat-labile, L-homoarginine- and levamisole-sensitive, and L-phenylalanine-insensitive (liver-bone-kidney type). These results show that vitamin D metabolites stimulate bone formation in vitro and that they may be involved in bone formation in vivo as well.

Original languageEnglish (US)
Pages (from-to)1127-1132
Number of pages6
JournalJournal of Biochemistry
Volume94
Issue number4
StatePublished - Oct 1983
Externally publishedYes

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Calcitriol
Cholecalciferol
Vitamins
Phosphatases
Bone
Skull
Alkaline Phosphatase
Mouse
Metabolites
Analogue
Osteogenesis
Vitamin D
Hormones
Cell
Homoarginine
Calcifediol
Bone and Bones
Levamisole
Phenylalanine
Kidney

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

1α,25-dihydroxyvitamin D3 and analogues of vitamin D3 induce alkaline phosphatase activity in osteoblastic cells derived from newborn mouse calvaria. / Haneji, Tatsuji; Kurihara, Noriyoshi; Ikeda, Katsumi; Kumegawa, Masayoshi.

In: Journal of Biochemistry, Vol. 94, No. 4, 10.1983, p. 1127-1132.

Research output: Contribution to journalArticle

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