2',3'-Dialdehyde of GTP blocks regulatory functions of adenylate cyclase Ns protein

Alexander Skurat, Maria S. Yurkova, Yuri V. Khropov, Tamara V. Bulargina, Eugene S. Severin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Preincubation of bovine caudate nucleus membranes with the 2',3'-dialdehyde of GTP (oGTP) reduces adenylate cyclase activation by guanylyl imidodiphosphate (GppNHp) in a time-dependent fashion. A slower rate of inhibition is observed if membranes are treated with both GTP and oGTP. The efficacy of oGTP action is enhanced by raising the Mg2+ concentration. Reduction of adenylate cyclase sensitivity to GppNHp is followed by an irreversible decrease of enzyme stimulation by forskolin. Addition of a Lubrol soluble preparation from guinea pig lung membranes to oGTP-treated caudate nucleus membranes causes restoration of the adenylate cyclase sensitivity to GppNHp. These data suggest that oGTP blocks the GTP-binding site of the adenylate cyclase system localized on the Ns protein. Such modification leads to the elimination of the Ns-mediated regulation of the enzyme.

Original languageEnglish (US)
Pages (from-to)150-154
Number of pages5
JournalFEBS Letters
Volume188
Issue number1
DOIs
StatePublished - Aug 19 1985
Externally publishedYes

Fingerprint

Guanosine Triphosphate
Adenylyl Cyclases
Membranes
Proteins
Caudate Nucleus
Guanylyl Imidodiphosphate
Colforsin
Enzymes
Restoration
2-(guanylformylmethoxy)-3-(triphospho)propanal
Guinea Pigs
Chemical activation
Binding Sites
Lung

Keywords

  • Adenylate cyclase
  • Caudate nucleus
  • Forskolin
  • GTP 2',3'-dialdehyde
  • N,protein
  • Reconstitution

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

2',3'-Dialdehyde of GTP blocks regulatory functions of adenylate cyclase Ns protein. / Skurat, Alexander; Yurkova, Maria S.; Khropov, Yuri V.; Bulargina, Tamara V.; Severin, Eugene S.

In: FEBS Letters, Vol. 188, No. 1, 19.08.1985, p. 150-154.

Research output: Contribution to journalArticle

Skurat, Alexander ; Yurkova, Maria S. ; Khropov, Yuri V. ; Bulargina, Tamara V. ; Severin, Eugene S. / 2',3'-Dialdehyde of GTP blocks regulatory functions of adenylate cyclase Ns protein. In: FEBS Letters. 1985 ; Vol. 188, No. 1. pp. 150-154.
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AU - Severin, Eugene S.

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