2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies

Silvia Marino, Ryan T. Bishop, Daniëlle de Ridder, Jesus Delgado-Calle, Michaela R. Reagan

Research output: Contribution to journalArticle

Abstract

Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.

Original languageEnglish (US)
Pages (from-to)71-98
Number of pages28
JournalMethods in molecular biology (Clifton, N.J.)
Volume1914
DOIs
StatePublished - Jan 1 2019

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Bone Neoplasms
Coculture Techniques
Cell Communication
Osteocytes
Osteoclasts
Osteoblasts
Adipocytes
Cell Culture Techniques
Bone Marrow
Gene Expression
In Vitro Techniques

Keywords

  • Cancer cells
  • In vitro co-culture
  • Osteoblasts
  • Osteoclasts
  • Osteocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies. / Marino, Silvia; Bishop, Ryan T.; de Ridder, Daniëlle; Delgado-Calle, Jesus; Reagan, Michaela R.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1914, 01.01.2019, p. 71-98.

Research output: Contribution to journalArticle

Marino, Silvia ; Bishop, Ryan T. ; de Ridder, Daniëlle ; Delgado-Calle, Jesus ; Reagan, Michaela R. / 2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies. In: Methods in molecular biology (Clifton, N.J.). 2019 ; Vol. 1914. pp. 71-98.
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