2D and 3D in vitro co-culture for cancer and bone cell interaction studies

Silvia Marino, Ryan T. Bishop, Daniëlle de Ridder, Jesus Delgado-Calle, Michaela R. Reagan

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages71-98
Number of pages28
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1914
ISSN (Print)1064-3745

Keywords

  • Cancer cells
  • In vitro co-culture
  • Osteoblasts
  • Osteoclasts
  • Osteocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Marino, S., Bishop, R. T., de Ridder, D., Delgado-Calle, J., & Reagan, M. R. (2019). 2D and 3D in vitro co-culture for cancer and bone cell interaction studies. In Methods in Molecular Biology (pp. 71-98). (Methods in Molecular Biology; Vol. 1914). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8997-3_5