2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies

Silvia Marino, Ryan T. Bishop, Daniëlle de Ridder, Jesus Delgado-Calle, Michaela R. Reagan

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.

Original languageEnglish (US)
Pages (from-to)71-98
Number of pages28
JournalMethods in molecular biology (Clifton, N.J.)
Volume1914
DOIs
StatePublished - Jan 1 2019

    Fingerprint

Keywords

  • Cancer cells
  • In vitro co-culture
  • Osteoblasts
  • Osteoclasts
  • Osteocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this