4-1BB ligand stimulation enhances myeloid dendritic cell maturation from human umbilical cord blood CD34+ progenitor cells

Young June Kim, Geling Li, Hal Broxmeyer

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In humans, at least two subsets of dendritic cells (DCs) are identified on the basis of differential surface expression of CD11c antigens. CD11c+ and CD11c- cells are respectively of myeloid and lympholoid origin and functionally distinct, eliciting inflammatory and tolerant T cell responses. We investigated whether 4-1BB ligand (4-1BBL), a member of the tumor necrosis factor (TNF) family, is involved in the maturation process to mature myeloid DCs during in vitro DC differentiation from immature DCs derived from human umbilical cord blood (CB) CD34+ progenitor cells. Enhanced levels of CD11c as well as immunostimulatory molecules such as CD86, MHC class II, and 4-1BBL were induced in response to 4-1BBL stimulation. These changes were accompanied by noticeable morphological transition from nonadherent to adherent myeloid-like DCs. Stimulation of 4-1BBL on DCs with 4-1BB-Fc or with 4-1BB-transfected Jurkat cells resulted in acquisition of capacity for the immature DCs to produce interleukin-12 (IL-12). This suggests that 4-1BBL may be an important mediator for maturation of CD11c+ myeloid DCs, information of possible relevance for the design of DC-based vaccines with enhanced activity.

Original languageEnglish
Pages (from-to)895-903
Number of pages9
JournalJournal of Hematotherapy and Stem Cell Research
Volume11
Issue number6
DOIs
StatePublished - Dec 2002

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4-1BB Ligand
Myeloid Cells
Fetal Blood
Dendritic Cells
Stem Cells
CD11c Antigens
Jurkat Cells
Interleukin-12
Cell Differentiation
Vaccines
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Hematology
  • Immunology

Cite this

4-1BB ligand stimulation enhances myeloid dendritic cell maturation from human umbilical cord blood CD34+ progenitor cells. / Kim, Young June; Li, Geling; Broxmeyer, Hal.

In: Journal of Hematotherapy and Stem Cell Research, Vol. 11, No. 6, 12.2002, p. 895-903.

Research output: Contribution to journalArticle

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