4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells

Young June Kim, Myung Kwan Han, Hal Broxmeyer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Ligation of NKG2D, a potent costimulatory receptor, can be either beneficial or detrimental to CD8+ cytotoxic T cell (CTL) responses. Factors for these diverse NKG2D effects remain elusive. In this study, we demonstrate that 4-1BB, another costimulatory receptor, is an essential regulator of NKG2D in CD8+ T cells. Costimulation of NKG2D caused down-modulation of NKG2D, but induced 4-1BB expression on the cell surface, even in the presence of TGF-β1, which inhibits 4-1BB expression. Resulting NKG2D-4-1BB+ cells were activated but still in an immature state with low cytotoxic activity. However, subsequent 4-1BB costimulation induced cytotoxic activity and restored down-modulated NKG2D. The cytotoxic activity and NKG2D expression induced by 4-1BB on NKG2D+4-1BB + cells were refractory to TGF-β1 down-modulation. Such 4-1BB effects were enhanced by IL-12. In contrast, in the presence of IL-4, 4-1BB effects were abolished because IL-4 down-modulated NKG2D and 4-1BB expression in cooperation with TGF-β1, generating another CD8+ T-cell type lacking both NKG2D and 4-1BB. These NKG2D-4-1BB- cells were inert and unable to gain cytotoxic activity. Our results suggest that 4-1BB plays a critical role in protecting NKG2D from TGF-β1-mediated down-modulation. Coexpression of NKG2D and 4-1BB may represent an important biomarker for defining competency of tumor infiltrating CD8+ T cells.

Original languageEnglish (US)
Pages (from-to)1378-1386
Number of pages9
JournalBlood
Volume111
Issue number3
DOIs
StatePublished - 2008

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T-cells
Fetal Blood
Blood
T-Lymphocytes
Modulation
Interleukin-4
Biomarkers
Interleukin-12
Refractory materials
Ligation
Tumors
Neoplasms

ASJC Scopus subject areas

  • Hematology

Cite this

4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells. / Kim, Young June; Han, Myung Kwan; Broxmeyer, Hal.

In: Blood, Vol. 111, No. 3, 2008, p. 1378-1386.

Research output: Contribution to journalArticle

Kim, Young June ; Han, Myung Kwan ; Broxmeyer, Hal. / 4-1BB regulates NKG2D costimulation in human cord blood CD8+ T cells. In: Blood. 2008 ; Vol. 111, No. 3. pp. 1378-1386.
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