46 kd mannose 6-phosphate receptor: Cloning, expression, and homology to the 215 kd mannose 6-phosphate receptor

Nancy M. Dahms, Peter Lobel, James Breitmeyer, John Chirgwin, Stuart Kornfeld

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

We have isolated cDNA clones encoding the entire sequence of the bovine 46 kd cation-dependent mannose 6-phosphate (CD Man-6-P) receptor. Translation of CD Man-6-P receptor mRNA in Xenopus laevis oocytes results in a protein that binds specifically to phosphomannan-Sepharose, thus demonstrating that our cDNA clones encode a functional receptor. The deduced 279 amino acid sequence reveals a single polypeptide chain that contains a putative signal sequence and a transmembrane domain. Trypsin digestion of microsomal membranes containing the receptor and the location of the five potential N-linked glycosylation sites indicate that the receptor is a transmembrane protein with an extracytoplasmic amino terminus. This extracytoplasmic domain is homologous to the approximately 145 amino acid long repeating domains present in the 215 kd cation-independent Man-6-P receptor.

Original languageEnglish (US)
Pages (from-to)181-192
Number of pages12
JournalCell
Volume50
Issue number2
DOIs
StatePublished - Jul 17 1987
Externally publishedYes

Fingerprint

IGF Type 2 Receptor
Cloning
Cations
Organism Cloning
Complementary DNA
Glycosylation
Amino Acids
Protein Sorting Signals
Clone Cells
Sepharose
Trypsin
Proteins
Xenopus laevis
Membranes
Messenger RNA
Peptides
Oocytes
Digestion
Amino Acid Sequence
mannose-6-phosphate

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

46 kd mannose 6-phosphate receptor : Cloning, expression, and homology to the 215 kd mannose 6-phosphate receptor. / Dahms, Nancy M.; Lobel, Peter; Breitmeyer, James; Chirgwin, John; Kornfeld, Stuart.

In: Cell, Vol. 50, No. 2, 17.07.1987, p. 181-192.

Research output: Contribution to journalArticle

Dahms, Nancy M. ; Lobel, Peter ; Breitmeyer, James ; Chirgwin, John ; Kornfeld, Stuart. / 46 kd mannose 6-phosphate receptor : Cloning, expression, and homology to the 215 kd mannose 6-phosphate receptor. In: Cell. 1987 ; Vol. 50, No. 2. pp. 181-192.
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