9 Mitochondrial α-ketoacid dehydrogenase kinases: A new family of protein kinases

Kirill M. Popov, John W. Hawes, Robert A. Harris

Research output: Contribution to journalArticle

39 Scopus citations


Four mitochondrial protein kinases have been cloned. These proteins represent a new family of protein kinases, related by sequence to the bacterial protein kinases but by function to the eukaryotic serine protein kinases. Arg288 is required for recognition by BCKDK of the phosphorylation site on the E1alpha subunit of the BCKDH complex. BCKDK inhibits the dehydrogenase activity of the BCKDH complex by introducing a negative charge into the active-site pocket of the E1 component. Protein starvation of rats induces an increase in the amount of BCKDK bound to the BCKDH complex. This causes inactivation of the BCKDH complex and conserves branched-chain amino acids for protein synthesis in the protein-starved state. Expression of the different PDK isoenzymes is tissue specific, and the different PDK isoenzymes are unique with respect to kinetic parameters for ATP and ADP and sensitivity to allosteric effectors (NADH, NAD+, coenzyme A, acetyl-CoA, pyruvate, and dichloroacetate). Preliminary experiments indicate that an increased amount of PDK2 protein partly explains the increase in PDK activity that occurs in rat liver in response to chemically induced diabetes.

Original languageEnglish (US)
Pages (from-to)105-111
Number of pages7
JournalAdvances in Second Messenger and Phosphoprotein Research
Issue numberC
StatePublished - Jan 1 1997

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of '9 Mitochondrial α-ketoacid dehydrogenase kinases: A new family of protein kinases'. Together they form a unique fingerprint.

  • Cite this