A 4.3 kb Smad7 promoter is able to specify gene expression during mouse development

Xubao Liu, Qian Chen, Chenzhong Kuang, Meiyu Zhang, Yiwen Ruan, Zao C. Xu, Zhenzhen Wang, Yan Chen

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Members of transforming growth factor-β (TGF-β) superfamily play important roles in diverse biological functions including early development. These extracellular factors exert their effects by interacting with membrane receptors followed by signal transduction by a group of Smad proteins. Smad7 is an inhibitory Smad protein that specifically antagonizes TGF-β and activin signaling. To characterize the developmental role of Smad7, a transgenic mouse model was generated using a 4.3 kb mouse Smad7 promoter driving β-galactosidase expression. In these mice, the Smad7 promoter defined a restrictive expression pattern of β-galactosidase in a tightly regulated temporal and spatial manner. The β-galactosidase gene was transiently expressed in the cardiovascular structures including heart cushion tissues and the endothelium of major arteries at E11.5 to E12.5. Through E12.5 to E17.5, β-galactosidase expression was prominently detected in the epithelium of developing cochlea and nasolacrimal duct. In addition, it was temporally expressed in trigeminal ganglion, the skeletal muscles surrounding major joints, primordium of the jaws, as well as genital tubercle. These studies indicated that the 4.3 kb Smad7 promoter contains sufficient regulatory elements to define controlled gene expression during mouse development.

Original languageEnglish (US)
Pages (from-to)149-152
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1769
Issue number2
DOIs
StatePublished - Feb 1 2007

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Keywords

  • Activin
  • Aorta
  • Cochlea
  • Development
  • Heart
  • Nasolacrimal duct
  • Smad7
  • TGF-β
  • Transgenic

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics
  • Medicine(all)

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