A biomarker panel for acute graft-versus-host disease

Sophie Paczesny, Oleg I. Krijanovski, Thomas M. Braun, Sung W. Choi, Shawn G. Clouthier, Rork Kuick, David E. Misek, Kenneth R. Cooke, Carrie L. Kitko, Angela Weyand, Daniel Bickley, Dawn Jones, Joel Whitfield, Pavan Reddy, John E. Levine, Samir M. Hanash, James L.M. Ferrara

Research output: Contribution to journalArticle

252 Scopus citations

Abstract

No validated biomarkers exist for acute graft-versus-host disease (GVHD). We screened plasma with antibody microarrays for 120 proteins in a discovery set of 42 patients who underwent transplantation that revealed 8 potential biomarkers for diagnostic of GVHD. We then measured by enzyme-linked immunosorbent assay (ELISA) the levels of these biomarkers in samples from 424 patients who underwent transplantation randomly divided into training (n = 282) and validation (n = 142) sets. Logistic regression analysis of these 8 proteins determined a composite biomarker panel of 4 proteins (interleukin-2-receptor- alpha, tumor-necrosis-factor-receptor-1, interleukin-8, and hepatocyte growth factor) that optimally discriminated patients with and without GVHD. The area under the receiver operating characteristic curve distinguishing these 2 groups in the training set was 0.91 (95% confidence interval, 0.87-0.94) and 0.86 (95% confidence interval, 0.79-0.92) in the validation set. In patients with GVHD, Cox regression analysis revealed that the biomarker panel predicted survival independently of GVHD severity. A panel of 4 biomarkers can confirm the diagnosis of GVHD in patients at onset of clinical symptoms of GVHD and provide prognostic information independent of GVHD severity.

Original languageEnglish (US)
Pages (from-to)273-278
Number of pages6
JournalBlood
Volume113
Issue number2
DOIs
StatePublished - Jan 8 2009

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Paczesny, S., Krijanovski, O. I., Braun, T. M., Choi, S. W., Clouthier, S. G., Kuick, R., Misek, D. E., Cooke, K. R., Kitko, C. L., Weyand, A., Bickley, D., Jones, D., Whitfield, J., Reddy, P., Levine, J. E., Hanash, S. M., & Ferrara, J. L. M. (2009). A biomarker panel for acute graft-versus-host disease. Blood, 113(2), 273-278. https://doi.org/10.1182/blood-2008-07-167098