A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

Nathan C. Shaner, Gerard G. Lambert, Andrew Chammas, Yuhui Ni, Paula J. Cranfill, Michelle A. Baird, Brittney R. Sell, John R. Allen, Richard N. Day, Maria Israelsson, Michael W. Davidson, Jiwu Wang

Research output: Contribution to journalArticle

377 Scopus citations


We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.

Original languageEnglish (US)
Pages (from-to)407-409
Number of pages3
JournalNature Methods
Issue number5
StatePublished - May 1 2013


ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Shaner, N. C., Lambert, G. G., Chammas, A., Ni, Y., Cranfill, P. J., Baird, M. A., Sell, B. R., Allen, J. R., Day, R. N., Israelsson, M., Davidson, M. W., & Wang, J. (2013). A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nature Methods, 10(5), 407-409. https://doi.org/10.1038/nmeth.2413