A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker

Linda H. Malkas, Brittney Shea Herbert, Waleed Abdel-Aziz, Lacey E. Dobrolecki, Yang Liu, Beamon Agarwal, Derek Hoelz, Sunil Badve, Lauren Schnaper, Randy J. Arnold, Yehia Mechref, Milos V. Novotny, Patrick Loehrer, Robert J. Goulet, Robert J. Mickey

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

Two isoforms of proliferating cell nuclear antigen (PCNA) have been observed in breast cancer cells. Commercially available antibodies to PCNA recognize both isoforms and, therefore, cannot differentiate between the PCNA isoforms in malignant and non-malignant breast epithelial cells and tissues. We have developed a unique antibody that specifically detects a PCNA isoform (caPCNA) associated with breast cancer epithelial cells grown in culture and breast-tumor tissues. Immunostaining studies using this antibody suggest that the caPCNA isoform may be useful as a marker of breast cancer and that the caPCNA-specific antibody could potentially serve as a highly effective detector of malignancy. We also report here that the caPCMA isoform functions in breast cancer-cell DNA replication and interacts with DNA polymerase S. Our studies indicate that the caPCMA isoform may be a previously uncharacterized detector of breast cancer.

Original languageEnglish (US)
Pages (from-to)19472-19477
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number51
DOIs
StatePublished - Dec 19 2006

Keywords

  • DNA replication
  • Genome stability
  • Mass spectrometry
  • Pathology
  • Posttranslational modification

ASJC Scopus subject areas

  • Genetics
  • General

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