Abstract
A cDNA clone which expresses a protein that cross-reacts immunologi-cally with the human Cl and C2 hnRNP core proteins has been isolated. The done was selected by a sensitive immunochemical assay employing an avidin-biotin complex for detection, and identified as a clone for the hnRNP C proteins by a highly sensitive antibody select assay that is described here. The clone contains 677 nucleotides, and, as shown by northern blotting, is derived from a 1.5 Kb poly(A)+ mRNA. There are regions of strong homology between the human and mouse genes, weak homology is seen with chicken DNA, and very little, if any, homology can be detected with Drosophila. Artemia. sea urchin, or yeast DNAs. Two peptides (a total of 24 amino acids) of the calf thymus single-stranded DNA binding protein UP2 show perfect homology with the deduced amino acid sequence of the clone, suggesting that UP2 is related to the hnRNP C proteins. There is also a region that has a sequence very similar to two regions of the single-stranded DNA binding protein UP1 that contain proposed DNA binding sites.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4077-4094 |
| Number of pages | 18 |
| Journal | Nucleic Acids Research |
| Volume | 14 |
| Issue number | 10 |
| DOIs | |
| State | Published - May 27 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Health, Toxicology and Mutagenesis
- Toxicology
- Genetics(clinical)
- Genetics
Cite this
A cDNA clone of the hnRNP C proteins and its homology with the single-stranded DNA binding protein UP2. / Lahiri, Debomoy; Thomas, John O.
In: Nucleic Acids Research, Vol. 14, No. 10, 27.05.1986, p. 4077-4094.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A cDNA clone of the hnRNP C proteins and its homology with the single-stranded DNA binding protein UP2
AU - Lahiri, Debomoy
AU - Thomas, John O.
PY - 1986/5/27
Y1 - 1986/5/27
N2 - A cDNA clone which expresses a protein that cross-reacts immunologi-cally with the human Cl and C2 hnRNP core proteins has been isolated. The done was selected by a sensitive immunochemical assay employing an avidin-biotin complex for detection, and identified as a clone for the hnRNP C proteins by a highly sensitive antibody select assay that is described here. The clone contains 677 nucleotides, and, as shown by northern blotting, is derived from a 1.5 Kb poly(A)+ mRNA. There are regions of strong homology between the human and mouse genes, weak homology is seen with chicken DNA, and very little, if any, homology can be detected with Drosophila. Artemia. sea urchin, or yeast DNAs. Two peptides (a total of 24 amino acids) of the calf thymus single-stranded DNA binding protein UP2 show perfect homology with the deduced amino acid sequence of the clone, suggesting that UP2 is related to the hnRNP C proteins. There is also a region that has a sequence very similar to two regions of the single-stranded DNA binding protein UP1 that contain proposed DNA binding sites.
AB - A cDNA clone which expresses a protein that cross-reacts immunologi-cally with the human Cl and C2 hnRNP core proteins has been isolated. The done was selected by a sensitive immunochemical assay employing an avidin-biotin complex for detection, and identified as a clone for the hnRNP C proteins by a highly sensitive antibody select assay that is described here. The clone contains 677 nucleotides, and, as shown by northern blotting, is derived from a 1.5 Kb poly(A)+ mRNA. There are regions of strong homology between the human and mouse genes, weak homology is seen with chicken DNA, and very little, if any, homology can be detected with Drosophila. Artemia. sea urchin, or yeast DNAs. Two peptides (a total of 24 amino acids) of the calf thymus single-stranded DNA binding protein UP2 show perfect homology with the deduced amino acid sequence of the clone, suggesting that UP2 is related to the hnRNP C proteins. There is also a region that has a sequence very similar to two regions of the single-stranded DNA binding protein UP1 that contain proposed DNA binding sites.
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U2 - 10.1093/nar/14.10.4077
DO - 10.1093/nar/14.10.4077
M3 - Article
VL - 14
SP - 4077
EP - 4094
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 10
ER -