A cDNA clone of the hnRNP C proteins and its homology with the single-stranded DNA binding protein UP2

Debomoy Lahiri, John O. Thomas

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A cDNA clone which expresses a protein that cross-reacts immunologi-cally with the human Cl and C2 hnRNP core proteins has been isolated. The done was selected by a sensitive immunochemical assay employing an avidin-biotin complex for detection, and identified as a clone for the hnRNP C proteins by a highly sensitive antibody select assay that is described here. The clone contains 677 nucleotides, and, as shown by northern blotting, is derived from a 1.5 Kb poly(A)+ mRNA. There are regions of strong homology between the human and mouse genes, weak homology is seen with chicken DNA, and very little, if any, homology can be detected with Drosophila. Artemia. sea urchin, or yeast DNAs. Two peptides (a total of 24 amino acids) of the calf thymus single-stranded DNA binding protein UP2 show perfect homology with the deduced amino acid sequence of the clone, suggesting that UP2 is related to the hnRNP C proteins. There is also a region that has a sequence very similar to two regions of the single-stranded DNA binding protein UP1 that contain proposed DNA binding sites.

Original languageEnglish (US)
Pages (from-to)4077-4094
Number of pages18
JournalNucleic Acids Research
Volume14
Issue number10
DOIs
StatePublished - May 27 1986
Externally publishedYes

Fingerprint

Heterogeneous-Nuclear Ribonucleoprotein Group C
DNA-binding Protein
DNA-Binding Proteins
CDNA
Clone
Homology
DNA
Complementary DNA
Clone Cells
Proteins
Protein
Assays
Heterogeneous-Nuclear Ribonucleoproteins
Amino Acids
Thymus
Avidin
Amino acids
Biotin
Artemia
Yeast

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

A cDNA clone of the hnRNP C proteins and its homology with the single-stranded DNA binding protein UP2. / Lahiri, Debomoy; Thomas, John O.

In: Nucleic Acids Research, Vol. 14, No. 10, 27.05.1986, p. 4077-4094.

Research output: Contribution to journalArticle

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