A compact blast-induced traumatic brain injury model in mice

Hongxing Wang, Yi Ping Zhang, Jun Cai, Lisa B E Shields, Chad A. Tuchek, Riyi Shi, Jianan Li, Christopher B. Shields, Xiao-Ming Xu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Blast-induced traumatic brain injury (bTBI) is a common injury on the battlefield and often results in permanent cognitive and neurological abnormalities. We report a novel compact device that creates graded bTBI in mice. The injury severity can be controlled by precise pressures that mimic Friedlander shockwave curves. The mouse head was stabilized with a head fixator, and the body was protected with a metal shield; shockwave durations were 3 to 4 milliseconds. Reflective shockwave peak readings at the position of the mouse head were 12 ± 2.6 psi, 50 ± 20.3 psi, and 100 ± 33.1 psi at 100, 200, and 250 psi predetermined driver chamber pressures, respectively. The bTBIs of 250 psi caused 80% mortality, which decreased to 27% with the metal shield. Brain and lung damage depended on the shockwave duration and amplitude. Cognitive deficits were assessed using the Morris water maze, Y-maze, and open-field tests. Pathological changes in the brain included disruption of the blood-brain barrier, multifocal neuronal and axonal degeneration, and reactive gliosis assessed by Evans Blue dye extravasation, silver and Fluoro-Jade B staining, and glial fibrillary acidic protein immunohistochemistry, respectively. Behavioral and pathological changes were injury severity-dependent. This mouse bTBI model may be useful for investigating injury mechanisms and therapeutic strategies associated with bTBI.

Original languageEnglish (US)
Pages (from-to)183-196
Number of pages14
JournalJournal of Neuropathology and Experimental Neurology
Volume75
Issue number2
DOIs
StatePublished - 2016

Fingerprint

Head
Wounds and Injuries
Metals
Pressure
Evans Blue
Gliosis
Glial Fibrillary Acidic Protein
Brain
Blood-Brain Barrier
Silver
Reading
Coloring Agents
Immunohistochemistry
Staining and Labeling
Equipment and Supplies
Lung
Mortality
Traumatic Brain Injury
Water
Therapeutics

Keywords

  • Behavioral assessments
  • Blast injury
  • Edema
  • Neuronal degeneration
  • Traumatic brain injury

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

A compact blast-induced traumatic brain injury model in mice. / Wang, Hongxing; Zhang, Yi Ping; Cai, Jun; Shields, Lisa B E; Tuchek, Chad A.; Shi, Riyi; Li, Jianan; Shields, Christopher B.; Xu, Xiao-Ming.

In: Journal of Neuropathology and Experimental Neurology, Vol. 75, No. 2, 2016, p. 183-196.

Research output: Contribution to journalArticle

Wang, H, Zhang, YP, Cai, J, Shields, LBE, Tuchek, CA, Shi, R, Li, J, Shields, CB & Xu, X-M 2016, 'A compact blast-induced traumatic brain injury model in mice', Journal of Neuropathology and Experimental Neurology, vol. 75, no. 2, pp. 183-196. https://doi.org/10.1093/jnen/nlv019
Wang, Hongxing ; Zhang, Yi Ping ; Cai, Jun ; Shields, Lisa B E ; Tuchek, Chad A. ; Shi, Riyi ; Li, Jianan ; Shields, Christopher B. ; Xu, Xiao-Ming. / A compact blast-induced traumatic brain injury model in mice. In: Journal of Neuropathology and Experimental Neurology. 2016 ; Vol. 75, No. 2. pp. 183-196.
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