A comparative assessment of the roles of CD8 and CD2 in the functions of activated murine CD8+ T lymphocytes

Astrid Reimann, Angelika Ehrfeld, Jörg Kupsch, Bernhard Maier, Kai M. Saizawa, Birgitta Clinchy, Klaus Eichmann

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Both CD8 and CD2 are T cell surface receptors involved in physical cell interaction and in transmembrane signalling. The present paper addresses their role in the induction of two different functions of the cloned murine cytotoxic T cell C196: target cell lysis and IFN-γ production. These functions were induced in C196 either by stimulation with the specific stimulator/target cell P815 or, bypassing specific recognition, by the aCD3 hybridoma 145-2C11 or by solid phase aTCR antibodies. These responses were tested for their susceptibility to inhibition/enhancement by a panel of aCD8 and aCD2 mAb. In addition, CD8 deficient and CD8/CD2 double-deficient variants of Cl96 were transfected with the CD8 and CD2 genes and the resulting cell lines were analysed for their functional capacities. The following results were obtained: (i) CD8 is primarily important in the specific recognition process of activated CTL; (ii) transmembrane signalling of activated CTL through the TCR does not require CD8, nor is it sensitive to modification through CD8; (iii) CTL can nevertheless be directly activated through CD8; however, this is restricted to induction of cytotoxicity but does not result in IFN-γ production; (iv) CD2 does not seem to be important in any of these responses.

Original languageEnglish (US)
Pages (from-to)149-158
Number of pages10
JournalImmunology Letters
Volume30
Issue number2
DOIs
StatePublished - Oct 1991

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Keywords

  • Accessory molecule
  • CD2
  • CD8
  • Cytotoxic T cell
  • IFN-γ
  • Specific target cell recognition
  • Transmembrane signalling

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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