A comparative study of spray keratinocytes and autologous meshed split-thickness skin graft in the treatment of acute burn injuries

Rajiv Sood, David Edward Roggy, Madeline Jane Zieger, Muhammad Nazim, Brett Colby Hartman, Jeff Thomas Gibbs

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Background. ReCell (Avita Medical, Northridge, CA) is an autologous cell harvesting (ACH) device that enables a thin splitthickness skin biopsy to be processed to produce a cell population that includes a mixed population of keratinocytes, melanocytes, Langerhans cells, and papillary dermal fibroblasts for immediate delivery via a spray applicator onto a prepared skin surface. Materials and Methods. In this Institutional Review Board-approved US Food and Drug Administration phase 2 study, the authors prospectively evaluated the treatment of partial-thickness burns in patients with two 320 cm2 areas, 1 area treated with the ACH device and the other with a meshed split-thickness skin graft (MSTSG) as a control. The authors compared the treatment areas for graft take, pigmentation, and color match to surrounding healthy tissue, scarring, and pain. Results. In this preliminary study, 10 patients were treated with this protocol. Eight patients had 100% take to both treatment areas and 2 patients had significant non-take and graft loss attributable to underexcised wound beds and difficulty with the spray applicator. Pigmentation and color match ratings were identical at week 52 and the Modified Vancouver Scar Scale scores were comparable. One subject rated the autologous cell harvesting site as having a better appearance, while the remaining subjects rated their ACH and MSTSG sites' appearances as being comparable. In early follow-up visits, pain ratings were slightly elevated in the ACH group due to graft healing; however, in visits following week 2, pain ratings at the ACH and MSTSG sites were rated similarly by all patients. Conclusion. This preliminary report describes an early experience with the ACH device and the treatment of partial-thickness burn injuries. In this 10-patient series, patients benefitted from having a decreased donor site size and comparable outcomes with MSTSG treatment. While this preliminary underpowered study has provided positive results, there is a learning curve with choosing the proper wound for treatment with the ACH device, as well as with using the device.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalWounds
Volume27
Issue number2
StatePublished - Feb 1 2015

Keywords

  • burn
  • skin graft
  • spray keratinocytes

ASJC Scopus subject areas

  • Surgery
  • Medical–Surgical

Fingerprint Dive into the research topics of 'A comparative study of spray keratinocytes and autologous meshed split-thickness skin graft in the treatment of acute burn injuries'. Together they form a unique fingerprint.

  • Cite this

    Sood, R., Roggy, D. E., Zieger, M. J., Nazim, M., Hartman, B. C., & Gibbs, J. T. (2015). A comparative study of spray keratinocytes and autologous meshed split-thickness skin graft in the treatment of acute burn injuries. Wounds, 27(2), 31-40.