A comparison of sarcoplasmic reticulum function in fast and slow skeletal muscle using crude homogenate and isolated vesicles

D. H. Kim, F. A. Witzmann, R. H. Fitts

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Sarcoplasmic reticulum vesicles (FSR) were isolated from relatively homogeneous muscle samples representative of type I, IIA, and IIB fibers and Ca2+ uptake (Vmax and total capacity) determined. Crude homogenates of these same fiber populations were also assayed for Ca2+ uptake and the results compared to the FSR values. Both techniques produced qualitatively similar results and demonstrated distinct fiber type differences in both the rate and extent of Ca2+ uptake. The results obtained support the contention that the crude homogenate technique accurately reflects the activity of the sarcoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)2223-2229
Number of pages7
JournalLife Sciences
Volume28
Issue number20
DOIs
StatePublished - May 18 1981
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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