A comparison of sarcoplasmic reticulum function in fast and slow skeletal muscle using crude homogenate and isolated vesicles

D. H. Kim, Frank Witzmann, R. H. Fitts

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Sarcoplasmic reticulum vesicles (FSR) were isolated from relatively homogeneous muscle samples representative of type I, IIA, and IIB fibers and Ca2+ uptake (Vmax and total capacity) determined. Crude homogenates of these same fiber populations were also assayed for Ca2+ uptake and the results compared to the FSR values. Both techniques produced qualitatively similar results and demonstrated distinct fiber type differences in both the rate and extent of Ca2+ uptake. The results obtained support the contention that the crude homogenate technique accurately reflects the activity of the sarcoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)2223-2229
Number of pages7
JournalLife Sciences
Volume28
Issue number20
DOIs
StatePublished - May 18 1981
Externally publishedYes

Fingerprint

Sarcoplasmic Reticulum
Muscle
Skeletal Muscle
Fibers
Muscles
Population

ASJC Scopus subject areas

  • Pharmacology

Cite this

A comparison of sarcoplasmic reticulum function in fast and slow skeletal muscle using crude homogenate and isolated vesicles. / Kim, D. H.; Witzmann, Frank; Fitts, R. H.

In: Life Sciences, Vol. 28, No. 20, 18.05.1981, p. 2223-2229.

Research output: Contribution to journalArticle

@article{489f526e26774c93a644f230155c2b5d,
title = "A comparison of sarcoplasmic reticulum function in fast and slow skeletal muscle using crude homogenate and isolated vesicles",
abstract = "Sarcoplasmic reticulum vesicles (FSR) were isolated from relatively homogeneous muscle samples representative of type I, IIA, and IIB fibers and Ca2+ uptake (Vmax and total capacity) determined. Crude homogenates of these same fiber populations were also assayed for Ca2+ uptake and the results compared to the FSR values. Both techniques produced qualitatively similar results and demonstrated distinct fiber type differences in both the rate and extent of Ca2+ uptake. The results obtained support the contention that the crude homogenate technique accurately reflects the activity of the sarcoplasmic reticulum.",
author = "Kim, {D. H.} and Frank Witzmann and Fitts, {R. H.}",
year = "1981",
month = "5",
day = "18",
doi = "10.1016/0024-3205(81)90573-7",
language = "English (US)",
volume = "28",
pages = "2223--2229",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "20",

}

TY - JOUR

T1 - A comparison of sarcoplasmic reticulum function in fast and slow skeletal muscle using crude homogenate and isolated vesicles

AU - Kim, D. H.

AU - Witzmann, Frank

AU - Fitts, R. H.

PY - 1981/5/18

Y1 - 1981/5/18

N2 - Sarcoplasmic reticulum vesicles (FSR) were isolated from relatively homogeneous muscle samples representative of type I, IIA, and IIB fibers and Ca2+ uptake (Vmax and total capacity) determined. Crude homogenates of these same fiber populations were also assayed for Ca2+ uptake and the results compared to the FSR values. Both techniques produced qualitatively similar results and demonstrated distinct fiber type differences in both the rate and extent of Ca2+ uptake. The results obtained support the contention that the crude homogenate technique accurately reflects the activity of the sarcoplasmic reticulum.

AB - Sarcoplasmic reticulum vesicles (FSR) were isolated from relatively homogeneous muscle samples representative of type I, IIA, and IIB fibers and Ca2+ uptake (Vmax and total capacity) determined. Crude homogenates of these same fiber populations were also assayed for Ca2+ uptake and the results compared to the FSR values. Both techniques produced qualitatively similar results and demonstrated distinct fiber type differences in both the rate and extent of Ca2+ uptake. The results obtained support the contention that the crude homogenate technique accurately reflects the activity of the sarcoplasmic reticulum.

UR - http://www.scopus.com/inward/record.url?scp=0019831726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019831726&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(81)90573-7

DO - 10.1016/0024-3205(81)90573-7

M3 - Article

C2 - 7253818

AN - SCOPUS:0019831726

VL - 28

SP - 2223

EP - 2229

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 20

ER -