A comparison of the inhibitory effects of taurine and GABA on identified Purkinje cells and other neurons in the cerebellar cortex of the rat

R. C A Frederickson, M. Neuss, Sandra Morzorati, W. J. McBride

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Abstract

The microiontophoretic application of taurine and GABA was studied in the cerebellar cortex of the rat. Both taurine and GABA produced a dose-dependent depression of spike frequency of cerebellar neurons. GABA (2-42 nA, mean 27 nA) induced an inhibition of spike discharge on all 138 cells tested, including 29 Purkinje cells. Taurine (60-200 nA, mean 108 nA) induced an inhibition of spike discharge on 93 of the 106 cerebellar neurons tested, including inhibition on 22 of 25 Purkinje cells. Iontophoretic application of bicuculline and picrotoxin antagonized the inhibitory effects of both GABA and taurine on Purkinje cells as well as on cerebellar neurons in general. Strychnine did not antagonize the inhibition of either GABA or taurine. Simultaneous application of taurine and GABA produced a synergistic inhibitory effect on the firing rate of Purkinje cells. Taurine, in contrast to GABA, appeared to be more depressant when applied in the Purkinje cell dendritic zone than when applied near the soma. The data are discussed in terms of taurine functioning as a neurotransmitter in the cerebellum of the rat and having receptor sites distinct from those for GABA.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalBrain Research
Volume145
Issue number1
DOIs
StatePublished - Apr 21 1978

Fingerprint

Cerebellar Cortex
Purkinje Cells
Taurine
gamma-Aminobutyric Acid
Neurons
Strychnine
Picrotoxin
Bicuculline
Carisoprodol
Cerebellum
Neurotransmitter Agents

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

A comparison of the inhibitory effects of taurine and GABA on identified Purkinje cells and other neurons in the cerebellar cortex of the rat. / Frederickson, R. C A; Neuss, M.; Morzorati, Sandra; McBride, W. J.

In: Brain Research, Vol. 145, No. 1, 21.04.1978, p. 117-126.

Research output: Contribution to journalArticle

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