A conserved heptamer upstream of the IgH promoter region octamer can be the site of a coordinate protein-DNA interaction

Nicholas F. Landolfi, Xiao-Ming Yin, J. Donald Capra, Philip W. Tucker

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Immunoglobulin genes contain a conserved eight base sequence element 5′ to the site of transcription initiation. This octamer can serve as a site for the binding of nuclear proteins which are presumably involved in the cell type specific expression of this family of genes. In studying the binding of nuclear proteins to this conserved sequence element, we have detected a protein interaction that involves, in addition to the octamer, nucleotides which are immediately upstream. We have characterized this additional contact as a sequence specific interaction with a heptameric sequence element (CTCATGA) that is conserved among Ig heavy chain promoters. Protein binding to the heptamer is unique in that it is dependent upon the proximity and orientation of, as well as protein interaction with, the conserved octamer.

Original languageEnglish (US)
Pages (from-to)5503-5514
Number of pages12
JournalNucleic Acids Research
Volume16
Issue number12
DOIs
StatePublished - Jun 24 1988
Externally publishedYes

Fingerprint

Nuclear Proteins
Genetic Promoter Regions
Promoter
Carrier Proteins
DNA
Genes
Proteins
Protein
Immunoglobulin Heavy Chains
Immunoglobulin Genes
Conserved Sequence
Transcription Initiation Site
Transcription
Nucleotides
Interaction
Protein Binding
Immunoglobulins
Binding Sites
Gene Expression
Gene

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

A conserved heptamer upstream of the IgH promoter region octamer can be the site of a coordinate protein-DNA interaction. / Landolfi, Nicholas F.; Yin, Xiao-Ming; Capra, J. Donald; Tucker, Philip W.

In: Nucleic Acids Research, Vol. 16, No. 12, 24.06.1988, p. 5503-5514.

Research output: Contribution to journalArticle

Landolfi, Nicholas F. ; Yin, Xiao-Ming ; Capra, J. Donald ; Tucker, Philip W. / A conserved heptamer upstream of the IgH promoter region octamer can be the site of a coordinate protein-DNA interaction. In: Nucleic Acids Research. 1988 ; Vol. 16, No. 12. pp. 5503-5514.
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