A continuous-flow method of organ culture

J. A. McAteer, O. D. Hegre

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A method of perfusion organ culture is described in which explants cultured at the air-medium interface are bathed by a continuous flow of nutrient medium. Morphological studies on the fetal rat lung indicate that explant development in this system is comparable to that obtained using standard organ-culture dishes. Medium supply is easily manipulated and continuous sampling of the effluent stream is possible without disturbing the immediate explant environment. The basic design facilitates secretory-response studies on cultured organ explants as demonstrated by a study of glucose-stimulated insulin release by the neonatal rat pancreas.

Original languageEnglish (US)
Pages (from-to)795-803
Number of pages9
JournalIn Vitro
Volume14
Issue number9
StatePublished - 1978
Externally publishedYes

Fingerprint

Organ Culture Techniques
Rats
Nutrients
Effluents
Pancreas
Perfusion
Air
Insulin
Sampling
Glucose
Food
Lung

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Developmental Biology

Cite this

McAteer, J. A., & Hegre, O. D. (1978). A continuous-flow method of organ culture. In Vitro, 14(9), 795-803.

A continuous-flow method of organ culture. / McAteer, J. A.; Hegre, O. D.

In: In Vitro, Vol. 14, No. 9, 1978, p. 795-803.

Research output: Contribution to journalArticle

McAteer, JA & Hegre, OD 1978, 'A continuous-flow method of organ culture', In Vitro, vol. 14, no. 9, pp. 795-803.
McAteer JA, Hegre OD. A continuous-flow method of organ culture. In Vitro. 1978;14(9):795-803.
McAteer, J. A. ; Hegre, O. D. / A continuous-flow method of organ culture. In: In Vitro. 1978 ; Vol. 14, No. 9. pp. 795-803.
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