A continuous-flow method of organ culture

James A. McAteer, Orion D. Hegre

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A method of perfusion organ culture is described in which explants cultured at the airmedium interface are bathed by a continuous flow of nutrient medium. Morphological studies on the fetal rat lung indicate that explant development in this system is comparable to that obtained using standard organ-culture dishes. Medium supply is easily manipulated and continuous sampling of the effluent stream is possible without disturbing the immediate explant environment. The basic design facilitates secretory-response studies on cultured organ explants as demonstrated by a study of glucose-stimulated insulin release by the neonatal rat pancreas.

Original languageEnglish (US)
Pages (from-to)795-803
Number of pages9
JournalIn Vitro: Journal of the Tissue Culture Association
Volume14
Issue number9
DOIs
StatePublished - Sep 1978

Keywords

  • fetal lung
  • insulin release
  • pancreas
  • perfusion organ culture

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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