A Correlation between Wnt/Beta-catenin Signaling and the Rate of Dentin Secretion

Yuan Zhao, Xue Yuan, Teresita Bellido, Jill A. Helms

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction: Odontoblasts produce dentin throughout life and in response to trauma. The purpose of this study was to identify the roles of endogenous Wnt signaling in regulating the rate of dentin accumulation. Methods: Histology, immunohistochemistry, vital dye labeling, and histomorphometric assays were used to quantify the rate of dentin accumulation as a function of age. Two strains of Wnt reporter mice were used to identify and follow the distribution and number of Wnt-responsive odontoblasts as a function of age. To show a causal relationship between dentin secretion and Wnt signaling, dentin accumulation was monitored in a strain of mice in which Wnt signaling was aberrantly elevated. Results: Dentin deposition occurs throughout life, but the rate of accumulation slows with age. This decline in dentin secretion correlates with a decrease in endogenous Wnt signaling. In a genetically modified strain of mice, instead of tubular dentin, aberrantly elevated Wnt signaling resulted in accumulation of reparative dentin or osteodentin secreted from predontoblasts. Conclusions: Wnt signaling regulates dentin secretion by odontoblasts, and the formation of reparative or osteodentin is the direct consequence of elevated Wnt signaling. These preclinical data have therapeutic implications for the development of a biologically based pulp capping medicant.

Original languageEnglish (US)
JournalJournal of Endodontics
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

beta Catenin
Dentin
Odontoblasts
Dental Pulp Capping
Histology
Coloring Agents
Immunohistochemistry
Wounds and Injuries

Keywords

  • Aging
  • odontoblasts
  • pulp
  • Wnt signal

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

A Correlation between Wnt/Beta-catenin Signaling and the Rate of Dentin Secretion. / Zhao, Yuan; Yuan, Xue; Bellido, Teresita; Helms, Jill A.

In: Journal of Endodontics, 01.01.2019.

Research output: Contribution to journalArticle

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