A creature with a hundred waggly tails: Intrinsically disordered proteins in the ribosome

Zhenling Peng, Christopher J. Oldfield, Bin Xue, Marcin J. Mizianty, A. Dunker, Lukasz Kurgan, Vladimir N. Uversky

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Intrinsic disorder (i.e., lack of a unique 3-D structure) is a common phenomenon, and many biologically active proteins are disordered as a whole, or contain long disordered regions. These intrinsically disordered proteins/regions constitute a significant part of all proteomes, and their functional repertoire is complementary to functions of ordered proteins. In fact, intrinsic disorder represents an important driving force for many specific functions. An illustrative example of such disorder-centric functional class is RNA-binding proteins. In this study, we present the results of comprehensive bioinformatics analyses of the abundance and roles of intrinsic disorder in 3,411 ribosomal proteins from 32 species. We show that many ribosomal proteins are intrinsically disordered or hybrid proteins that contain ordered and disordered domains. Predicted globular domains of many ribosomal proteins contain noticeable regions of intrinsic disorder. We also show that disorder in ribosomal proteins has different characteristics compared to other proteins that interact with RNA and DNA including overall abundance, evolutionary conservation, and involvement in protein-protein interactions. Furthermore, intrinsic disorder is not only abundant in the ribosomal proteins, but we demonstrate that it is absolutely necessary for their various functions.

Original languageEnglish
Pages (from-to)1477-1504
Number of pages28
JournalCellular and Molecular Life Sciences
Volume71
Issue number8
DOIs
StatePublished - 2014

Fingerprint

Intrinsically Disordered Proteins
Ribosomes
Ribosomal Proteins
Proteins
RNA-Binding Proteins
Proteome
Computational Biology
RNA
DNA

Keywords

  • Intrinsically disordered protein
  • Moonlighting protein
  • Protein-protein interaction
  • Protein-RNA interaction
  • Ribosomal proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Pharmacology
  • Cellular and Molecular Neuroscience

Cite this

A creature with a hundred waggly tails : Intrinsically disordered proteins in the ribosome. / Peng, Zhenling; Oldfield, Christopher J.; Xue, Bin; Mizianty, Marcin J.; Dunker, A.; Kurgan, Lukasz; Uversky, Vladimir N.

In: Cellular and Molecular Life Sciences, Vol. 71, No. 8, 2014, p. 1477-1504.

Research output: Contribution to journalArticle

Peng, Zhenling ; Oldfield, Christopher J. ; Xue, Bin ; Mizianty, Marcin J. ; Dunker, A. ; Kurgan, Lukasz ; Uversky, Vladimir N. / A creature with a hundred waggly tails : Intrinsically disordered proteins in the ribosome. In: Cellular and Molecular Life Sciences. 2014 ; Vol. 71, No. 8. pp. 1477-1504.
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