A facile enzymatic technique for the estimation of nanomolar concentrations of N-phosphonacetyl-l-aspartic acid in plasma

Thomas W. Kensler, Hiremagalur N. Jayaram, David A. Cooney

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A sensitive radiometric enzyme-inhibition assay is described for the determination of N-phosphonacetyl-l acid (PALA, NSC-224,131) in plasma. The assay is based on the inhibition by PALA, at low concentrations of the competitive co-substrate carbamyl phosphate, of L-aspartate transcarbamylase prepared from mouse spleen. The assay responds with good reproducibility and in proportional way to concentrations of PALA over the range of 2-20 nM; as such, it represents a 100-fold increase in sensitivity over existing methodologies. The applicability and pertinence of this assay to long-term pharmacokinetic studies with PALA is demonstrated by the one month persistence of nanomolar concentrations of PALA in the plasma of mice treated with a single dose of the drug.

Original languageEnglish (US)
Pages (from-to)29-35
Number of pages7
JournalJournal of Biochemical and Biophysical Methods
Volume2
Issue number1-2
DOIs
StatePublished - Jan 1 1980

Keywords

  • N-phosphonacetyl-l-aspartic acid (PALA)
  • PALA assay
  • PALA pharmaco-kinetics
  • l-aspartate transcarbamylase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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