A facile radiometric technique for measuring ATP

David A. Cooney, H. N. Jayaram, Elton R. Homan, Charles F. Motley

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A facile radiometric technique for measuring ATP in samples of biologic origin is presented. The d-glucose-6-phosphate produced by the phosphorylation of excess tritiated d-glucose with crystalline hexokinase is stoichiometrically equivalent to the ATP present. Product is selectively precipitated with ethanolic barium acetate, washed with ethanol and the tritium label counted in a scintillation spectrometer. The technique is especially suitable for the measurement of ATP in large numbers of samples (50-100) and offers acceptable sensitivity down to 62 fmoles.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalAnalytical biochemistry
Volume62
Issue number1
DOIs
StatePublished - Nov 1974

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cooney, D. A., Jayaram, H. N., Homan, E. R., & Motley, C. F. (1974). A facile radiometric technique for measuring ATP. Analytical biochemistry, 62(1), 157-165. https://doi.org/10.1016/0003-2697(74)90376-5