A facile radiometric technique for measuring ATP

David A. Cooney, H. N. Jayaram, Elton R. Homan, Charles F. Motley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A facile radiometric technique for measuring ATP in samples of biologic origin is presented. The d-glucose-6-phosphate produced by the phosphorylation of excess tritiated d-glucose with crystalline hexokinase is stoichiometrically equivalent to the ATP present. Product is selectively precipitated with ethanolic barium acetate, washed with ethanol and the tritium label counted in a scintillation spectrometer. The technique is especially suitable for the measurement of ATP in large numbers of samples (50-100) and offers acceptable sensitivity down to 62 fmoles.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalAnalytical Biochemistry
Volume62
Issue number1
DOIs
StatePublished - 1974
Externally publishedYes

Fingerprint

Adenosine Triphosphate
Glucose-6-Phosphate
Scintillation counters
Phosphorylation
Hexokinase
Tritium
Barium
Labels
Acetates
Ethanol
Crystalline materials
Glucose

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cooney, D. A., Jayaram, H. N., Homan, E. R., & Motley, C. F. (1974). A facile radiometric technique for measuring ATP. Analytical Biochemistry, 62(1), 157-165. https://doi.org/10.1016/0003-2697(74)90376-5

A facile radiometric technique for measuring ATP. / Cooney, David A.; Jayaram, H. N.; Homan, Elton R.; Motley, Charles F.

In: Analytical Biochemistry, Vol. 62, No. 1, 1974, p. 157-165.

Research output: Contribution to journalArticle

Cooney, DA, Jayaram, HN, Homan, ER & Motley, CF 1974, 'A facile radiometric technique for measuring ATP', Analytical Biochemistry, vol. 62, no. 1, pp. 157-165. https://doi.org/10.1016/0003-2697(74)90376-5
Cooney, David A. ; Jayaram, H. N. ; Homan, Elton R. ; Motley, Charles F. / A facile radiometric technique for measuring ATP. In: Analytical Biochemistry. 1974 ; Vol. 62, No. 1. pp. 157-165.
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